State Key Laboratory of Microbial Resources , Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101 , P. R. China.
Department of Chemistry and Biochemistry , University of California , Los Angeles California 90095 , United States.
J Am Chem Soc. 2019 Nov 6;141(44):17517-17521. doi: 10.1021/jacs.9b10217. Epub 2019 Oct 23.
A dedicated enzyme for the formation of the central C ring in the tetracyclic ergoline of clinically important ergot alkaloids has never been found. Herein, we report a dual role catalase (EasC), unexpectedly using O as the oxidant, that catalyzes the oxidative cyclization of the central C ring from a 1,3-diene intermediate. Our study showcases how nature evolves the common catalase for enantioselective C-C bond construction of complex polycyclic scaffolds.
一种用于形成临床上重要的麦角生物碱四环麦角灵中环 C 的专用酶从未被发现。在此,我们报告了一种双功能过氧化氢酶(EasC),出人意料地使用 O 作为氧化剂,催化中环 C 环从 1,3-二烯中间体的氧化环化。我们的研究展示了自然界如何进化出常见的过氧化氢酶,用于构建复杂的多环支架的对映选择性 C-C 键。