巴西犊牛和秘鲁骆驼科动物粪便中冠状病毒的分子流行病学
Molecular epidemiology of coronavirus in faeces of Brazilian calves and Peruvian camelid herds.
作者信息
Rocha Camila B, Fornells Luz Alba A M G, Rojas Miguel, Libetal Maíra, Manchego Alberto, Pezo Danilo, Santos Norma
机构信息
Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Empresa de Pesquisa Agropecuária do Estado do Rio de Janeiro, Niterói, RJ, Brazil.
出版信息
J Infect Dev Ctries. 2018 Jan 31;12(1):37-42. doi: 10.3855/jidc.9528.
INTRODUCTION
The enteric disorders represent a serious hazard for bovine and camelid breeding. The aim of this study was to examine the frequency of detection and molecular characteristics of enteric coronavirus (CoV) infections in cattle, alpaca, and llama herds bred in family-based farms in Brazil and Peru.
METHODOLOGY
Stool samples were collected from calves from Brazil and camelids from Peru for detection and characterization of CoV by reverse transcription polymerase chain reaction (RT-PCR) and sequence analysis.
RESULTS
46.5% (47/101) samples from calves and 26.8% (70/261) from alpaca tested positive for CoV. All strains belong to lineage A1 of the Betacoronavirus genus. Phylogenetic analysis showed high identity between CoV strains detected in calves and alpacas.
CONCLUSIONS
This study characterised CoV strains from dairy cattle herds in the state of Rio de Janeiro, Brazil, and indicated that this virus is spread among the state herds. The results also indicate widespread circulation of CoV among the alpacas of Cuzco, Peru.
引言
肠道疾病对牛和骆驼科动物养殖构成严重危害。本研究的目的是检测巴西和秘鲁家庭式农场养殖的牛群、羊驼群和骆马群中肠道冠状病毒（CoV）感染的检出频率及分子特征。
方法
采集来自巴西的犊牛和来自秘鲁的骆驼科动物的粪便样本，通过逆转录聚合酶链反应（RT-PCR）和序列分析检测和鉴定CoV。
结果
犊牛样本中46.5%（47/101）检测出CoV呈阳性，羊驼样本中26.8%（70/261）检测出CoV呈阳性。所有毒株均属于β冠状病毒属的A1分支。系统发育分析表明，在犊牛和羊驼中检测到的CoV毒株之间具有高度同源性。
结论
本研究对巴西里约热内卢州奶牛群中的CoV毒株进行了特征分析，并表明该病毒在该州牛群中传播。结果还表明CoV在秘鲁库斯科的羊驼中广泛传播。
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