Molecular characterization of isolated from cheese and biocontrol of Shiga toxigenic with essential oils.

The current research was carried out to study the incidence of li () in Egyptian cheese (Kariesh and Ras) and molecular characterization of certain virulence genes () using multiplex PCR technique. Biocontrol of with essential oils (clove and thyme oil) was also studied. A total of 150 random samples of Kariesh and Ras cheese (75 each) were collected from various areas in Governorate of Menoufia. According to our results, the frequency of isolated from Kariesh and Ras cheese was 16% and 5.3%, respectively. Serological identification classified the strains into two groups, enterohemorrhagic (EHEC) serogroup (O26: H11, O91: H21, O111: H2 and O103: H2). While the enterotoxigenic (ETEC) serogroup were detected as O125: H21 which is the most prevalent strain. O171: H2, O86 and O119: H6 belonging to enteropathogenic (EPEC). The most prevalent gene detected in strains was (87.5%) followed by (86%), (75%), (50%) and (25%) genes. Concerning the antimicrobial activity with essential oils, thyme oil (1%) is considered as the bactericidal effect against (ATCC35150) with improved the sensory evaluation than clove oil (1%). In conclusion, Kariesh and Ras cheese are extremely tainted with pathogenic strains, which represent a strong hazard on the human health.