Lara Valéria Maria, Carregaro Adriano Bonfim, Santurio Deise Flores, de Sá Mariangela Facco, Santurio Janio Moraes, Alves Sydney Hartz
Department of Veterinary Medicine, University of São Paulo, Avenida Duque de Caxias Norte 225, 13635-900 Pirassununga, SP, Brazil.
Department of Microbiology and Parasitology, Federal University of Santa Maria, Avenida Roraima 1000, 97105-900 Santa Maria, RS, Brazil.
Evid Based Complement Alternat Med. 2016;2016:1643762. doi: 10.1155/2016/1643762. Epub 2016 May 30.
This study evaluated the in vitro antibacterial activity of essential oils from Lippia graveolens (Mexican oregano), Origanum vulgaris (oregano), Thymus vulgaris (thyme), Rosmarinus officinalis (rosemary), Cymbopogon nardus (citronella), Cymbopogon citratus (lemongrass), and Eucalyptus citriodora (eucalyptus) against Escherichia coli (n = 22) strains isolated from Alouatta spp. feces. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined for each isolate using the broth microdilution technique. Essential oils of Mexican oregano (MIC mean = 1818 μg mL(-1); MBC mean = 2618 μg mL(-1)), thyme (MIC mean = 2618 μg mL(-1); MBC mean = 2909 μg mL(-1)), and oregano (MIC mean = 3418 μg mL(-1); MBC mean = 4800 μg mL(-1)) showed the best antibacterial activity, while essential oils of eucalyptus, rosemary, citronella, and lemongrass displayed no antibacterial activity at concentrations greater than or equal to 6400 μg mL(-1). Our results confirm the antimicrobial potential of some essential oils, which deserve further research.
本研究评估了墨西哥牛至（Lippia graveolens）、牛至（Origanum vulgaris）、百里香（Thymus vulgaris）、迷迭香（Rosmarinus officinalis）、香茅（Cymbopogon nardus）、柠檬草（Cymbopogon citratus）和柠檬桉（Eucalyptus citriodora）的精油对从蛛猴属粪便中分离出的22株大肠杆菌的体外抗菌活性。使用肉汤微量稀释技术测定每种分离株的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。墨西哥牛至精油（MIC平均值 = 1818 μg mL⁻¹；MBC平均值 = 2618 μg mL⁻¹）、百里香精油（MIC平均值 = 2618 μg mL⁻¹；MBC平均值 = 2909 μg mL⁻¹）和牛至精油（MIC平均值 = 3418 μg mL⁻¹；MBC平均值 = 4800 μg mL⁻¹）显示出最佳抗菌活性，而柠檬桉、迷迭香、香茅和柠檬草的精油在浓度大于或等于6400 μg mL⁻¹时未显示出抗菌活性。我们的结果证实了某些精油的抗菌潜力，值得进一步研究。
