Zou You, Shao Linzhi, Wu Yingxuan
Guangdong Inspection and Quarantine Technology Center, Guangzhou 510623, China.
Se Pu. 2019 Oct 8;37(10):1112-1117. doi: 10.3724/SP.J.1123.2019.03031.
A method was developed for the simultaneous determination of 11 benzimildazoles residue markers in chicken meat, liver and kidney samples by high performance liquid chromatography (HPLC). The chicken tissue sample was extracted with acetonitrile, defatted with -hexane, and purified with an MCX solid-phase extraction cartridge. Chromatographic separation was performed on an Atlantis T column with the gradient elution of methyl alcohol-1% (v/v) acetic acid aqueous solution as the mobile phases at a flow rate of 1.0 mL/min. The detection wavelength was set at 292 nm. Analytes were quantified by the external standard method. The linearities of the calibration curves were well in the range of 25-1000 μg/L, and the limit of quantification was 50 μg/kg. The recoveries at different spiked levels in all the three matrices ranged from 70.91% to 103.21%, and the relative standard deviations (RSDs) were between 1.48% and 10.08% (=6). The developed method is characterized by an excellent purification effect, sensitivity and accuracy, and can be used for the determination of benzimidazoles residue markers in chicken tissue samples.
建立了一种用高效液相色谱法（HPLC）同时测定鸡肉、肝脏和肾脏样品中11种苯并咪唑类残留标志物的方法。鸡肉组织样品用乙腈提取，用正己烷脱脂，并用MCX固相萃取柱净化。在Atlantis T柱上进行色谱分离，以甲醇-1%（v/v）乙酸水溶液为流动相进行梯度洗脱，流速为1.0 mL/min。检测波长设定为292 nm。采用外标法对分析物进行定量。校准曲线在25-1000 μg/L范围内线性良好，定量限为50 μg/kg。在所有三种基质中不同加标水平下的回收率为70.91%至103.21%，相对标准偏差（RSD）在1.48%至10.08%之间（n=6）。所建立的方法具有良好的净化效果、灵敏度和准确性，可用于鸡肉组织样品中苯并咪唑类残留标志物的测定。
