Faculty of Ceilandia, University of Brasilia, Brasilia 72220-900, Brazil.
Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, Brazil.
J Mater Chem B. 2019 Oct 23;7(41):6390-6398. doi: 10.1039/c9tb00587k.
The aim of this work was to develop and test the in vitro biological activity of nanocapsules loaded with a doxorubicin (DOX) free base dissolved in a core of castor oil shelled by poly(methyl vinyl ether-co-maleic anhydride) conjugated to n-octadecylamine residues. This system was stable and monodisperse, with a hydrodynamic diameter of about 300 nm. These nanocapsules changed the intracellular distribution of DOX, from the nuclei to the cytoplasm, and exhibited higher toxicity towards cancer cells - 4T1 and MCF-7 - and significantly lower toxicity towards normal cells - NIH-3T3 and MCF-10A - in vitro. In conclusion, these nanocapsules are suitable DOX carriers, which remain to be studied in in vivo tumor models.
这项工作的目的是开发和测试载有游离阿霉素（DOX）的纳米胶囊的体外生物活性，该 DOX 溶解在由聚（甲基乙烯基醚-马来酸酐）接枝到正十八烷基胺残基的壳聚糖油核中。该系统稳定且单分散，水动力直径约为 300nm。这些纳米胶囊改变了 DOX 的细胞内分布，从细胞核到细胞质，并表现出对癌细胞（4T1 和 MCF-7）更高的毒性，而对正常细胞（NIH-3T3 和 MCF-10A）的毒性显著降低。总之，这些纳米胶囊是合适的 DOX 载体，有待在体内肿瘤模型中进一步研究。
