Hixson D C, Miller M F, Maruyama K, Walborg E F, Wagner S, Starling J J, Bowen J M
J Histochem Cytochem. 1979 Dec;27(12):1618-29. doi: 10.1177/27.12.316439.
Statistical procedures were utilized to evaluate the concentration dependence of labeling by ferritin-conjugated lectins on four different rat cells: hepatocytes, normal thymocytes, Friend virus-induced rat tumor cells and feline sarcoma virus-induced rat sarcoma cells. Labeling by ferritin conjugates of concanavalin A, wheat germ agglutinin and Ricinus communis agglutinins I and II was quantitated by counting the number of ferritin granules on 600 Angstrom membrane segments. Relationships between the arithmetic means and variances for sample populations from each cell and ferritin-lectin combination were used to define four types of topographical distributions: uniform/ordered, uniform/random, random and clustered. It was found that the distribution and/or density of surface-bound lectin was concentration-dependent for all four ferritin-lectins. The nature of this dependency was complex and varied with both lectin and cell type.
运用统计学方法评估了铁蛋白结合凝集素对四种不同大鼠细胞（肝细胞、正常胸腺细胞、弗瑞德病毒诱导的大鼠肿瘤细胞和猫肉瘤病毒诱导的大鼠肉瘤细胞）标记的浓度依赖性。通过计数600埃膜段上的铁蛋白颗粒数量，对伴刀豆球蛋白A、麦胚凝集素以及蓖麻凝集素I和II的铁蛋白缀合物标记进行了定量。利用每个细胞与铁蛋白-凝集素组合的样本群体的算术平均值和方差之间的关系，定义了四种类型的拓扑分布：均匀/有序、均匀/随机、随机和聚集。结果发现，对于所有四种铁蛋白-凝集素，表面结合凝集素的分布和/或密度均具有浓度依赖性。这种依赖性的性质很复杂，并且因凝集素和细胞类型而异。
