通过测定ASC寡聚化来检测P2X7嘌呤受体激活引发的炎性小体激活
Detection of Inflammasome Activation by P2X7 Purinoceptor Activation by Determining ASC Oligomerization.
作者信息
Martínez-Banaclocha Helios, Pelegrín Pablo
机构信息
Biomedical Research Institute of Murcia (IMIB), Clinical University Hospital Virgen de la Arrixaca, Murcia, Spain.
出版信息
Methods Mol Biol. 2020;2041:335-343. doi: 10.1007/978-1-4939-9717-6_25.
The purinoceptor P2X7 is highly expressed in cells of the innate immune system, including monocytes and macrophages. Its activation is a potent signal to activate the NLRP3 inflammasome and induce the release of proinflammatory cytokines of the IL-1 family. In this chapter, we present a method to monitor NLRP3 inflammasome activation in human monocytes upon P2X7 receptor stimulation by detecting intracellular oligomers of ASC by flow cytometry. This method could be used to evaluate the degree of inflammasome activation in blood samples from patients suffering different chronic inflammatory diseases.
嘌呤受体P2X7在先天性免疫系统的细胞中高度表达，包括单核细胞和巨噬细胞。其激活是激活NLRP3炎性小体并诱导IL-1家族促炎细胞因子释放的有效信号。在本章中，我们介绍一种通过流式细胞术检测ASC的细胞内寡聚体来监测P2X7受体刺激后人单核细胞中NLRP3炎性小体激活的方法。该方法可用于评估患有不同慢性炎症性疾病患者血液样本中炎性小体的激活程度。
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