Department of Planning and Coordination, National Livestock Breeding Center, Fukushima, Japan.
Division of Pathology and Pathophysiology, National Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki, Japan.
Anim Sci J. 2019 Dec;90(12):1523-1529. doi: 10.1111/asj.13303. Epub 2019 Oct 23.
We aimed to define whether embryo collection carried out after pseudopregnancy was of similar outcome and quality as after artificial abortion. To induce pseudopregnancy, 30 gilts or sows were given 20 mg intramuscular estradiol dipropionate (EDP) 10-11 days after the onset of estrus. Ten additional pigs were inseminated artificially at natural estrus as a control group. Prostaglandin F (PGF ) was administered twice with a 24 hr interval beginning 15, 20, or 25 days after EDP-treatment (n = 10 per group) or between 23 and 39 days after artificial insemination in control pigs. Following this, all pigs were given 1,000 IU equine chorionic gonadotropin and 500 IU human chorionic gonadotropin (hCG) and then inseminated. Embryos were recovered 6 or 7 days after hCG treatment and outcome was recorded. There was no significant difference in the number of normal embryos collected from the pigs with PGF initiated at different time points or from the control group. Embryonic developmental stages 7 days after hCG treatment also did not differ among groups. These results indicate that the use of EDP to induce pseudopregnancy, followed by PGF administration to synchronize estrus for subsequent embryo harvest, is a suitable alternative to the artificial abortion method.
我们旨在确定在假妊娠后进行的胚胎采集是否与人工流产后的结果和质量相似。为了诱导假妊娠，在发情开始后 10-11 天，给 30 头母猪或公猪肌内注射 20mg 二丙酸雌二醇（EDP）。另外 10 头猪在自然发情时进行人工授精作为对照组。PGF 在 EDP 处理后 15、20 或 25 天（每组 10 头）或人工授精后 23 至 39 天（对照组）之间每隔 24 小时两次给予前列腺素 F（PGF），然后所有猪都给予 1000IU 马绒毛膜促性腺激素和 500IU 人绒毛膜促性腺激素（hCG），然后进行授精。hCG 处理后 6 或 7 天回收胚胎并记录结果。从不同时间点开始用 PGF 处理的猪或对照组中收集的正常胚胎数量没有显著差异。hCG 处理后 7 天胚胎发育阶段在各组之间也没有差异。这些结果表明，使用 EDP 诱导假妊娠，然后用 PGF 给药同步发情以随后进行胚胎收获，是人工流产方法的合适替代方法。
