Dillner L, Dickerson K, Manthorpe M, Ruoslahti E, Engvall E
Cancer Research Center, La Jolla Cancer Research Foundation, California 92037.
Exp Cell Res. 1988 Jul;177(1):186-98. doi: 10.1016/0014-4827(88)90036-5.
The interaction of cells with laminin and laminin fragments was studied in short-term cell attachment assays. Neurite-promoting chymotrypsin fragments of laminin were isolated using a monoclonal antibody which blocks neurite outgrowth on laminin. The fragments were shown, by electron microscopy after rotary shadowing and by immunological reactivity with different monoclonal antibodies, to contain only the distal end of the long arm. These fragments promoted the attachment and spreading of glioma, sarcoma, carcinoma, muscle, and endodermal cells to the same extent as intact laminin. The attachment was unaffected by peptides containing the RGD sequence. The morphology of the cells on the chymotrypsin fragments was indistinguishable from that on intact laminin but different from the morphology of the same cells on fibronectin. Light microscopy and scanning electron microscopy showed extensive process formation on laminin but not on fibronectin suggestive of increased cell motility in response to laminin. We conclude that the neurite-promoting domain of laminin contains a major site of interaction for non-neuronal cells and that this site induces a cellular response in certain non-neuronal cells that is unique to laminin.
在短期细胞附着实验中研究了细胞与层粘连蛋白及层粘连蛋白片段的相互作用。使用一种可阻断层粘连蛋白上神经突生长的单克隆抗体，分离出了具有促神经突生长作用的层粘连蛋白胰凝乳蛋白酶片段。经旋转投影后的电子显微镜观察以及与不同单克隆抗体的免疫反应性显示，这些片段仅包含长臂的远端部分。这些片段促进胶质瘤、肉瘤、癌、肌肉和内胚层细胞附着与铺展的程度与完整的层粘连蛋白相同。附着不受含RGD序列的肽的影响。胰凝乳蛋白酶片段上细胞的形态与完整层粘连蛋白上细胞的形态无法区分，但与同一细胞在纤连蛋白上的形态不同。光学显微镜和扫描电子显微镜显示，层粘连蛋白上有广泛的突起形成，而纤连蛋白上则没有，这表明层粘连蛋白可诱导细胞运动增加。我们得出结论，层粘连蛋白的促神经突生长结构域包含非神经元细胞相互作用的主要位点，并且该位点在某些非神经元细胞中诱导出一种层粘连蛋白特有的细胞反应。
