Kanclerz A, Wiebe L I, Luu K, Knaus E E
Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Canada.
J Nucl Med. 1988 Jul;29(7):1252-8.
The biodistribution of i.v. administered [67Ga]citrate was investigated in the Lewis lung carcinoma/male B6D2F1 mouse model. Tumors were implanted intramuscularly (10(5) cells or 10(6) cells in suspension) into the thigh, or subcutaneously (10(7) cells or 2 mm3 fragments) into the tail of recipient mice. Intramuscular tumors were allowed to grow for 16, 24, or 33 days; tail tumors developed for 2 wk (fragment implants) or 3 wk (10(7) cells in suspension) after which the primary tumor was amputated along with adjacent fragments of the tail tissue. Gamma camera scintigraphy and dissection/radiometric biodistribution studies indicated that: (a) tumors and metastases took up 5-6% of the injected dose/g except when large necrotic areas were present in the primary tumor; (b) blood levels of 67Ga increased in all tumor-bearing animals, with up to tenfold increases in the i.m. tumor model at later stages of the growth; (c) hepatic uptake increased as a function of tumor size/age, and (d) all tissue:blood ratios declined as the neoplastic tissues progressed. The results are discussed with respect to tumor progression and metastatic disease.
在Lewis肺癌/雄性B6D2F1小鼠模型中研究了静脉注射[67Ga]柠檬酸盐的生物分布。将肿瘤（10⁵个细胞或10⁶个悬浮细胞）肌肉注射到受体小鼠的大腿中，或将肿瘤（10⁷个细胞或2立方毫米组织块）皮下植入受体小鼠的尾巴。肌肉肿瘤生长16、24或33天；尾巴肿瘤在植入组织块后生长2周或在植入10⁷个悬浮细胞后生长3周，之后将原发肿瘤连同尾巴组织的相邻组织块一起切除。γ相机闪烁扫描以及解剖/放射性生物分布研究表明：（a）肿瘤和转移灶摄取的放射性剂量为每克注射剂量的5 - 6%，但原发肿瘤存在大片坏死区域时除外；（b）所有荷瘤动物的血液中67Ga水平均升高，在肌肉肿瘤模型生长后期升高可达10倍；（c）肝脏摄取随肿瘤大小/年龄增加而增加，且（d）随着肿瘤组织进展，所有组织与血液的比值均下降。针对肿瘤进展和转移性疾病对结果进行了讨论。
