College of Food Science and Technology/College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu, P. R. China.
Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology/Key Laboratory for Control Technology and Standard for Agro-product Safety and Quality, Ministry of Agriculture and Rural Affairs/Key Laboratory for Agro-product Safety Risk Evaluation (Nanjing), Ministry of Agriculture and Rural Affairs/Collaborative Innovation Center for Modern Grain Circulation and Safety/Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, P. R. China.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2020 Jan;37(1):143-152. doi: 10.1080/19440049.2019.1678768. Epub 2019 Oct 24.
B-type fumonisins (FBs) are water-soluble mycotoxins produced by species, which are mainly found in maize products and threaten food safety. Toxicological studies and quantitative determinations of fumonisins require large amounts of pure toxins, and their high prices limit progress in FBs research. In this study, we used a macroporous resin column combined with high-speed countercurrent chromatography to separate large quantities of FBs. A fermented rice culture was extracted with 75% methanol. The dynamic adsorption capacity of FBs on XAD-2 resin was 27.5 mg/g resin at 25°C, pH 4.0, and then the FBs were desorbed with 60% methanol. The crude FBs were further purified using a biphasic system consisting of n-heptane/n-butanol/methanol/water (2:4:1:4, v/v/v/v). The method yielded 1.55 g of FB1 and 0.55 g of FB3 with purities of 96.8% and 95.6%, respectively, from 1 kg of rice culture, and the final overall yield of FBs was 74.8%.
B 型伏马菌素（FBs）是由 种产生的水溶性真菌毒素，主要存在于玉米制品中，威胁着食品安全。毒理学研究和伏马菌素的定量测定需要大量的纯毒素，而其高昂的价格限制了 FBs 研究的进展。在本研究中，我们使用大孔树脂柱结合高速逆流色谱法分离大量的 FBs。发酵大米培养物用 75%甲醇提取。在 25°C、pH 值 4.0 时，FBs 在 XAD-2 树脂上的动态吸附容量为 27.5mg/g 树脂，然后用 60%甲醇解吸。粗 FB 进一步用正庚烷/正丁醇/甲醇/水（2:4:1:4，v/v/v/v）两相体系纯化。该方法从 1 公斤大米培养物中得到 1.55 克 FB1 和 0.55 克 FB3，纯度分别为 96.8%和 95.6%，FBs 的最终总收率为 74.8%。
