细胞培养中使用稳定同位素标记代谢前体的 PTM 动态的质谱分析。

Mass spectrometric analysis of PTM dynamics using stable isotope labeled metabolic precursors in cell culture.

机构信息

Department of Analytical Biochemistry, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.

出版信息

Analyst. 2019 Nov 18;144(23):6812-6833. doi: 10.1039/c9an01258c.

DOI:10.1039/c9an01258c

PMID:31650141

Abstract

Biological organisms represent highly dynamic systems, which are continually exposed to environmental factors and always strive to restore steady-state homeostasis. Posttranslational modifications are key regulators with which biological systems respond to external stimuli. To understand how homeostasis is restored, it is important to study the kinetics of posttranslational modifications. In this review we discuss proteomic approaches using stable isotope labeled metabolic precursors to study dynamics of posttranslational modifications in cell culture.

摘要

生物有机体代表着高度动态的系统，它们不断暴露于环境因素之中，并始终努力恢复稳定的内稳态。翻译后修饰是生物系统对外界刺激做出响应的关键调节剂。为了了解内稳态是如何恢复的，研究翻译后修饰的动力学是很重要的。在这篇综述中，我们讨论了使用稳定同位素标记代谢前体的蛋白质组学方法来研究细胞培养中翻译后修饰的动力学。