Institute of Chemistry, University of Silesia in Katowice, 75 Pułku Piechoty 1, 41-500, Chorzów, Poland.
A. Chełkowski Institute of Physics and Silesian Center for Education and Interdisciplinary Research, University of Silesia in Katowice, 75 Pułku Piechoty 1, 41-500, Chorzów, Poland.
Sci Rep. 2019 Oct 25;9(1):15304. doi: 10.1038/s41598-019-50734-8.
A novel pro-dye approach for the acid-selective staining of the subcellular compartments for better permeability and selectivity was applied. The designed sensor has suitable physicochemical properties such as a large Stokes shift and a long-lived intracellular fluorescence. The Schiff base fragment was used for the acid-sensitive release of a fluorophore without affecting the overall stability of the biological systems. Due to the presence of an imine bond in its structure and its unique fluorescent properties, it can be presented as a "pro-dye" for acidic structures such as lysosomes. As a result of an imine bond cleavage, a new fluorescent compound is released, whose substantially shifted excitation and emission wavelengths enable a more selective and effective imaging of lysosomes and endosomes. The presented report provides the chemical, physicochemical and optical profiles as well as biological assays and theoretical calculations.
一种新的前染料方法被应用于亚细胞区室的酸选择性染色，以提高通透性和选择性。设计的传感器具有合适的物理化学性质，如大斯托克斯位移和长寿命的细胞内荧光。席夫碱片段用于荧光团的酸敏感释放，而不影响生物系统的整体稳定性。由于其结构中存在亚胺键及其独特的荧光性质，它可以作为溶酶体等酸性结构的“前染料”。由于亚胺键的断裂，释放出一种新的荧光化合物，其显著位移的激发和发射波长能够更选择性和有效地对溶酶体和内体进行成像。本报告提供了化学、物理化学和光学特性以及生物测定和理论计算。
