Chanratana Mak, Joe Manoharan Melvin, Roy Choudhury Aritra, Anandham Rangasamy, Krishnamoorthy Ramasamy, Kim Kiyoon, Jeon Sunyoung, Choi Joonho, Choi Jeongyun, Sa Tongmin
1Department of Environmental and Biological Chemistry, Chungbuk National University, Cheongju, Chungbuk Republic of Korea.
6Department of Plant Protection Sanitary and Phytosanitary, General Directorate of Agriculture, Ministry of Agriculture, Forestry and Fisheries, Phnom Penh, Cambodia.
3 Biotech. 2019 Nov;9(11):397. doi: 10.1007/s13205-019-1923-1. Epub 2019 Oct 11.
The use of plant growth promoting bacteria as bioinoculant to alleviate salt stress is a sustainable and eco-friendly approach in agriculture. However, the maintenance of the bacterial population in the soil for longer period is a major concern. In the present study, chitosan-immobilized aggregated CBMB20 was used as a bioinoculant to improve tomato plant ( Mill.) growth under salt stress. The chitosan-immobilized aggregated CBMB20 was able to enhance plant dry weight, nutrient uptake (N, P, K and Mg), photosynthetic efficiency and decrease electrolyte leakage under salt stress conditions. The oxidative stress exerted by elevated levels of salt stress was also alleviated by the formulated bioinoculant, as it up-regulated the antioxidant enzyme activities and enhanced the accumulation of proline which acts as an osmolyte. The chitosan-immobilized aggregated CBMB20 was able to decrease the excess Na influx into the plant cells and subsequently decreasing the Na/K ratio to improve tomato plant growth under salt stress conditions. Therefore, it is proposed that the chitosan-immobilized aggregated . CBMB20 could be used as a bioinoculant to promote the plant growth under salt stress conditions.
利用植物促生细菌作为生物菌剂来缓解盐胁迫是农业中一种可持续且环保的方法。然而，如何使细菌群体在土壤中长时间维持是一个主要问题。在本研究中，壳聚糖固定化聚集的CBMB20被用作生物菌剂，以改善盐胁迫下番茄植株（Mill.）的生长。壳聚糖固定化聚集的CBMB20能够提高植株干重、养分吸收（氮、磷、钾和镁）、光合效率，并在盐胁迫条件下减少电解质渗漏。配制的生物菌剂还缓解了盐胁迫水平升高所施加的氧化胁迫，因为它上调了抗氧化酶活性，并增强了作为渗透调节剂的脯氨酸的积累。壳聚糖固定化聚集的CBMB20能够减少过量的钠流入植物细胞，并随后降低钠/钾比，以改善盐胁迫条件下番茄植株的生长。因此，建议壳聚糖固定化聚集的CBMB20可作为生物菌剂用于促进盐胁迫条件下的植物生长。
