Kim Kiyoon, Kyaw Ei Phyu, Ramasamy Krishnamoorthy, Walitang Denver I, Rajasekaran Raghu
National Forest Seed Variety Center, Chungju 27495, Republic of Korea.
Department of Biotechnology Research, Ministry of Science and Technology, Kyaukse 05151, Mandalay Region, Myanmar.
J Microbiol Biotechnol. 2025 Jul 11;35:e2501007. doi: 10.4014/jmb.2501.01007.
Farming communities are very concerned about salt stress because of its negative impact on crop productivity. This study evaluated the ability of the CBMB20 and exogenous trehalose treatments on growth under salt stress conditions. growth was enhanced using CBMB20 as a bioinoculant in both saline and non-saline environments. In addition to better photosynthetic efficiency and endogenous trehalose content, the inoculation of CBMB20 produced improved growth parameters, such as increased rosette fresh weight and shoot length. Reduced levels of proline and malondialdehyde (MDA) under salt stress (150 mM NaCl) further indicated that the inoculated plants had enhanced tolerance to salinity. GFP-tagged CBMB20 was also used in spatial distribution experiments, which showed that the bacteria colonized 's root, shoot, and hypocotyl. By increasing shoot length and total fresh weight, the exogenous application of trehalose also markedly enhanced plant growth. Proline and MDA contents were decreased by exogenous trehalose during salt stress, while the endogenous trehalose concentration in remained unaffected. The use of trehalose and CBMB20 can both have a good impact on plant development and stress tolerance in saline environments.
农业社区非常关注盐胁迫,因为它会对作物生产力产生负面影响。本研究评估了CBMB20和外源海藻糖处理在盐胁迫条件下对生长的影响。在盐渍和非盐渍环境中,使用CBMB20作为生物接种剂均可促进生长。除了具有更好的光合效率和内源海藻糖含量外,接种CBMB20还产生了改善的生长参数,如莲座鲜重增加和茎长增加。在盐胁迫(150 mM NaCl)下脯氨酸和丙二醛(MDA)水平降低,进一步表明接种的植物对盐分的耐受性增强。带有绿色荧光蛋白(GFP)标签的CBMB20也用于空间分布实验,结果表明该细菌定殖在植物的根、茎和下胚轴中。通过增加茎长和总鲜重,外源施用海藻糖也显著促进了植物生长。在盐胁迫期间,外源海藻糖降低了脯氨酸和MDA含量,而植物体内的内源海藻糖浓度未受影响。在盐渍环境中,使用海藻糖和CBMB20均可对植物发育和胁迫耐受性产生良好影响。