Gopal Geetha, Roy Namrata, Chandrasekaran Natarajan, Mukherjee Amitava
Centre for NanoBiotechnology, VIT, Vellore 632014, Tamil Nadu, India.
ACS Omega. 2019 Oct 7;4(17):17504-17510. doi: 10.1021/acsomega.9b02339. eCollection 2019 Oct 22.
In the present study, we report an efficient method for tetracycline (TC) removal from contaminated wastewater using alginate beads, immobilized with bio nanocomposite (BNC) consisting of FeO (iron oxide) and TiO (titanium dioxide) nanoparticles along with dead biomass of TC-resistant bacteria sp. Chemically synthesized FeO nanoparticles and commercially available TiO (P) nanoparticles were combined to form nanocomposite followed by encapsulation within alginate beads along with heat-killed biomass of sp. for the efficient degradation and adsorption of the target pollutant. The primary characterization of chemically synthesized nanoparticles was carried out with Fourier transform infrared, scanning electron microscopy-energy-dispersive X-ray spectrometry, transmission electron microscopy, and X-ray diffraction techniques. The batch studies for TC removal were performed by varying the reaction parameters such as bead weight, initial TC concentration, and pH in a photoreactor with UV-C irradiation. TC concentration of 10 mg/L, bead weight 10 g, and pH 6 were fixed as the optimum condition where 98 ± 0.5% of TC was removed from the solution. The possible removal mechanism was investigated with the help of UV-visible, total organic carbon, oxidation-reduction potential, Brunauer-Emmett-Teller, and liquid chromatography-mass spectroscopy analyses. The applicability of the process was successfully tested with the natural water systems spiked with TC at 10 mg/L. To assess the ecotoxic effects of the treated effluents, the cell viability assay was performed with the algal strains, , and sp. and the bacterial strains, and . Finally, the reusability of the BNC bead was successfully established up to the 4th cycle.
在本研究中，我们报道了一种从受污染废水中去除四环素（TC）的有效方法，该方法使用藻酸盐珠，其固定有由FeO（氧化铁）和TiO（二氧化钛）纳米颗粒以及耐TC细菌菌株的死生物质组成的生物纳米复合材料（BNC）。化学合成的FeO纳米颗粒和市售的TiO（P）纳米颗粒结合形成纳米复合材料，然后与菌株的热灭活生物质一起封装在藻酸盐珠中，以有效降解和吸附目标污染物。通过傅里叶变换红外光谱、扫描电子显微镜-能量色散X射线光谱、透射电子显微镜和X射线衍射技术对化学合成的纳米颗粒进行了初步表征。通过在具有UV-C照射的光反应器中改变反应参数，如珠重量、初始TC浓度和pH值，进行了TC去除的批次研究。将10 mg/L的TC浓度、10 g的珠重量和pH 6固定为最佳条件，在此条件下从溶液中去除了98±0.5%的TC。借助紫外可见光谱、总有机碳、氧化还原电位、布鲁诺尔-埃米特-泰勒比表面积测定法和液相色谱-质谱分析研究了可能的去除机制。该工艺的适用性已成功地在添加了10 mg/L TC的天然水系统中进行了测试。为了评估处理后废水溶液的生态毒性效应，对藻类菌株、和菌株以及细菌菌株和进行了细胞活力测定。最后，成功确定了BNC珠在第4个循环之前的可重复使用性。
