Department of Women's Health Tübingen , Calwerstraße 7 , 72076 Tübingen , Germany.
Natural and Medical Sciences Institute (NMI) , Reutlingen , Germany.
ACS Appl Mater Interfaces. 2019 Nov 20;11(46):42885-42895. doi: 10.1021/acsami.9b13221. Epub 2019 Nov 11.
Noninvasive epithelial tissue treatment with cold atmospheric plasma (CAP) is a promising option for local treatment of chronic inflammatory and precancerous lesions as well as various mucosal cancer diseases. Atmospheric pressure plasma jets (APPJ) are well-characterized and medically approved plasma sources. There are numbers of medically approved plasma sources for the treatment of epithelial diseases; however, little is known about the biochemical effects of CAP at the plasma-tissue interface. Furthermore, the actual penetration depth of CAP into tissue is currently unclear. Noninvasive and marker-independent Raman microspectroscopy was employed to assess the molecular effects of CAP on single cells and primary human cervical tissue samples. CAP treatment showed immediate and persisting changes of specific molecular tissue components determined by multivariate analysis. Raman imaging identified CAP-dependent changes in the morphology of the tissue, as well as molecular tissue components. The expression of the different components was not significantly altered within 24 h of incubation. DNA and lipids showed the strongest changes upon CAP treatment, which were traced to the basal cell layer of cervical epithelium, corresponding to an average functional plasma penetration depth of roughly 270 μm. In this study, Raman microspectroscopy is shown to be a promising method for molecular single-cell and solid tissue characterization. Regarding CAP treatment of tissues, Raman microspectroscopy could be suitable for the screening of biological mechanisms as well as for future contact- and marker-independent monitoring of plasma tissue effects.
利用冷等离体大气（CAP）对非侵入性上皮组织进行治疗，是局部治疗慢性炎症和癌前病变以及各种黏膜癌疾病的一种很有前途的选择。大气压等离子体射流（APPJ）是一种特征明确且已获得医学认可的等离子体源。有许多已获得医学认可的等离子体源可用于治疗上皮疾病；然而，人们对 CAP 在等离子体-组织界面的生化作用知之甚少。此外，CAP 进入组织的实际穿透深度目前尚不清楚。本研究采用非侵入性且无需标记的拉曼显微光谱技术，评估 CAP 对单细胞和人原发性宫颈组织样本的分子影响。多元分析表明，CAP 处理会立即并持续改变特定的分子组织成分。拉曼成像可识别 CAP 依赖性的组织形态和分子组织成分变化。在孵育 24 小时内，不同成分的表达没有明显改变。CAP 处理后 DNA 和脂质的变化最为明显，这些变化可追溯到宫颈上皮的基底层，对应于平均约 270 μm 的功能等离子体穿透深度。本研究表明，拉曼显微光谱技术是一种用于分子单细胞和固体组织特性分析的很有前途的方法。就 CAP 对组织的治疗而言，拉曼显微光谱技术可能适合于筛选生物机制，以及未来用于接触式和无标记的等离子体-组织相互作用的监测。
