Palomino Jesus Manuel, Mastromonaco Gabriela F, Cervantes Miriam P, Mapletoft Reuben J, Anzar Muhammad, Adams Gregg P
Department of Biomedical Sciences, University of Guelph, Guelph, ON, Canada.
Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, SK, Canada.
Reprod Domest Anim. 2020 Jan;55(1):54-63. doi: 10.1111/rda.13580. Epub 2019 Dec 13.
Two experiments were done using a two-by-two design to determine the effects of season and superstimulatory protocol on embryo production in wood bison. In Experiment 1 (in vivo-derived embryos), ovarian superstimulation was induced in female bison during the ovulatory and anovulatory seasons with either two or three doses of FSH given every-other-day (FSH × 2 vs. FSH × 3, respectively). Bison were given hCG to induce ovulation, inseminated 12 and 24 hr after hCG, and embryos were collected 8 days after hCG (n = 10 bison/group). In Experiment 2 (in vitro embryo production), ovarian superstimulation was induced in female bison during the ovulatory and anovulatory seasons with two doses of FSH, and in vivo maturation of the cumulus-oocyte complexes (COC) was induced with hCG at either 48 or 72 hr after the last dose of FSH. COC were collected 34 hr after hCG, and expanded COC were used for in vitro fertilization and culture. In Experiment 1, the number of follicles ≥9 mm, the proportion of follicles that ovulated, the number of CL, and the total number of ova/embryos collected did not differ between seasons or treatment groups, but the number of transferable embryos was greater (p < .05) in the ovulatory season. In Experiment 2, no differences were detected between seasons or treatment groups for any end point. The number of transferable embryos produced per bison was greatest (p < .05) using in vitro fertilization and was unaffected by season (1.5 ± 0.2 and 1.1 ± 0.3 during anovulatory and ovulatory seasons, respectively), in contrast to in vivo embryo production which was affected by season (0.1 ± 0.01 and 0.7 ± 0.2 during anovulatory and ovulatory seasons, respectively). Results demonstrate that transferable embryos can be produced throughout the year in wood bison by both in vivo and in vitro techniques, but the efficiency of embryo production of in vivo-derived embryos is significantly lower during the anovulatory season.
进行了两项实验，采用二乘二设计来确定季节和超刺激方案对林地野牛胚胎生产的影响。在实验1（体内来源胚胎）中，在排卵季节和非排卵季节，对雌性野牛进行卵巢超刺激，每隔一天给予两剂或三剂促卵泡素（分别为FSH×2和FSH×3）。给野牛注射人绒毛膜促性腺激素（hCG）以诱导排卵，在注射hCG后12小时和24小时进行授精，并在注射hCG后8天收集胚胎（每组n = 10头野牛）。在实验2（体外胚胎生产）中，在排卵季节和非排卵季节，对雌性野牛进行卵巢超刺激，给予两剂促卵泡素，并在最后一剂促卵泡素后的48小时或72小时，用hCG诱导卵丘-卵母细胞复合体（COC）的体内成熟。在注射hCG后34小时收集COC，并将扩张的COC用于体外受精和培养。在实验1中，≥9毫米卵泡的数量、排卵卵泡的比例、黄体的数量以及收集到的卵子/胚胎总数在不同季节或处理组之间没有差异，但可移植胚胎的数量在排卵季节更多（p < 0.05）。在实验2中，任何终点指标在不同季节或处理组之间均未检测到差异。与受季节影响的体内胚胎生产（非排卵季节和排卵季节分别为0.1±0.01和0.7±0.2）相比，使用体外受精时，每头野牛产生的可移植胚胎数量最多（p < 可移植胚胎数量不受季节影响（非排卵季节和排卵季节分别为1.5±0.2和1.1±0.3）。结果表明，通过体内和体外技术，林地野牛全年均可生产可移植胚胎，但在非排卵季节，体内来源胚胎的生产效率显著较低。 05），且
