Apiwong K, Wongsawad C H, Butboonchoo P
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.
The Applied Technology for Biodiversity Research Unit, Institute for Science and Technology, Chiang Mai University, Chiang Mai, Thailand 50200, Thailand.
Helminthologia. 2018 Oct 27;55(4):334-342. doi: 10.2478/helm-2018-0034. eCollection 2018 Dec.
Cyprinoid fish in Chiang Mai province has been reported the presence of a large number of metacercariae, particularly the metacercariae of and those not identified to species. This study aims to investigate morphological and molecular characteristic of the minute intestinal fluke metacercariae in two cyprinoid fish species from Chom Thong district, Chiang Mai province, Thailand: the Tinfoil barb () and the White eye barb (). A total of 180 fish (90 from and 90 from . ) were collected over three seasons: cool, hot and the rainy season (December 2015 to August 2016). Fish were examined for metacercariae infection, including areas such as muscle and the inner side of body scales, by using a light microscope. The prevalence of metacercariae in and was 73.33 % and 100 % respectively. metacercariae were identified as based on the morphological characteristics; the position of the acetabulum and the number and arrangement of the acetabular spines. Phylogenetic analysis based on Cytochrome c Oxidase subunit I (COI) gene showed that metacercariae from and were the same species as the adult stage of from and . Both morphological and molecular characteristic could indicate that metacercariae originated from this study were . Furthermore, it is a new record of the minute intestinal fluke in Chiang Mai Province.
据报道，清迈府的鲤科鱼类体内存在大量尾蚴，尤其是未鉴定到种的尾蚴。本研究旨在调查泰国清迈府Chom Thong区两种鲤科鱼类（锡箔鲃（）和白眼鲃（））体内微小肠吸虫尾蚴的形态和分子特征。在三个季节（2015年12月至2016年8月的凉爽季、炎热季和雨季）共收集了180条鱼（90条锡箔鲃和90条白眼鲃）。通过光学显微镜检查鱼体的肌肉和鳞片内侧等部位是否感染尾蚴。锡箔鲃和白眼鲃体内尾蚴的感染率分别为73.33%和100%。根据形态特征，包括吸盘的位置以及吸盘棘的数量和排列，将尾蚴鉴定为。基于细胞色素c氧化酶亚基I（COI）基因的系统发育分析表明，锡箔鲃和白眼鲃体内的尾蚴与来自的成虫阶段为同一物种。形态和分子特征均表明，本研究中获得的尾蚴为。此外，这是清迈府微小肠吸虫的新记录。
