Mačák Kubašková T, Mudroňová D, Gergeľ-Čechová M, Hrčková G
Institute of Parasitology of the Slovak Academy of Sciences, Hlinkova 3, 04001 Košice, Slovak Republic.
Institute of Microbiology and Immunology, The University of Veterinary Medicine and Pharmacy, Komenského 57, 041 81 Košice, Slovak Republic.
Helminthologia. 2019 Sep 1;56(3):183-195. doi: 10.2478/helm-2019-0021. eCollection 2019 Sep.
The metacestode stage of the tapeworm () has the ability of asexual growth in the peritoneal cavity of rodents and other intermediate hosts without restriction. Early immunological events have decisive role in the establishment of infection. In the present study we investigated the kinetic of myeloid and lymphoid cell populations and the proportions of cells undergoing apoptosis in peritoneal cavities of mice within the first month after oral infection with larvae. Proportions of cell phenotypes and apoptotic cells were examined by flow cytometry and by microscopical analysis of cells following May/Grünwald staining and fluorescent stain Hoechst 33234, respectively. Total numbers of peritoneal cells increased and their distribution changed towards accumulation of myelo-monocytic cell lineage in the account of reduced proportions of lymphoid cells. CD4+ T cell subpopulations were more abundant than CD8+ and their proportions elevated within two weeks post infection (p.i.) which was followed by a significant decline. Expression level of CD11c marker on myelo-monocytic cells revealed phenotype heterogeneity and proportions of cells with low and medium expression elevated from day 14 p.i. along with concurrent very low presence of CD11c phenotype. Lymphoid cell population was highly resistant to apoptosis but elevated proportions of myeloid cells were in early/late stage of apoptosis. Apoptosis was detected in a higher number of adherent cells from day 14 p.i. onwards as evidenced by nuclear fluorescent staining. By contrast, cells adherent to larvae, mostly macrophages and eosinophils, did not have fragmented nuclei. Our data demonstrated that apoptosis did not account for diminished population of peritoneal lymphoid cells and substantial proportions of myeloid cells seem to be more susceptible to apoptotic turnover in peritoneal cavity of mice with ongoing infection, suggesting their important role in the host-parasite interactions.
绦虫（ ）的中绦期在啮齿动物和其他中间宿主的腹腔内具有无限制的无性生长能力。早期免疫事件在感染的建立中起决定性作用。在本研究中，我们调查了口服感染 幼虫后第一个月内小鼠腹腔中髓样和淋巴样细胞群体的动力学以及发生凋亡的细胞比例。分别通过流式细胞术以及对经May/Grünwald染色和荧光染料Hoechst 33234染色后的细胞进行显微镜分析，来检测细胞表型和凋亡细胞的比例。腹腔细胞总数增加，其分布朝着髓系单核细胞谱系积累的方向变化，这是由于淋巴细胞比例降低所致。CD4 + T细胞亚群比CD8 +更丰富，并且在感染后两周内其比例升高，随后显著下降。髓系单核细胞上CD11c标志物的表达水平显示出表型异质性，从感染后第14天起，低表达和中等表达细胞的比例升高，同时CD11c表型的存在非常低。淋巴样细胞群体对凋亡具有高度抗性，但髓样细胞比例升高处于凋亡的早期/晚期。从感染后第14天起，通过核荧光染色证明，在大量贴壁细胞中检测到凋亡。相比之下，附着在幼虫上的细胞，主要是巨噬细胞和嗜酸性粒细胞，没有细胞核碎片化。我们的数据表明，凋亡并不能解释腹腔淋巴细胞数量的减少，并且在持续感染 的小鼠腹腔中，相当比例的髓样细胞似乎更容易发生凋亡更新，这表明它们在宿主 - 寄生虫相互作用中起重要作用。
