七氟醚通过改善缺血再灌注后离体大鼠心脏的TDR和MAPD来减轻再灌注心律失常。
Sevoflurane Alleviates Reperfusion Arrhythmia by Ameliorating TDR and MAPD in Isolated Rat Hearts after Ischemia-Reperfusion.
作者信息
Wang Guilong, Dai Dongjun, Gao Hong, Liu Yanqiu, Wang Zijun, Li Huayu, Fu Xiaokui
机构信息
School of Anesthesiology, Guizhou Medical University, Guiyang, Guizhou, China.
The Third Affiliated Hospital of Guizhou Medical University, Duyun, Guizhou, China.
出版信息
Anesthesiol Res Pract. 2019 Sep 22;2019:7910930. doi: 10.1155/2019/7910930. eCollection 2019.
OBJECTIVE
To investigate the effect of sevoflurane on the monophasic action potentials (MAPs) in isolated rat hearts after ischemia-reperfusion.
METHODS
Twenty-four healthy SD male rats, weighing 280-320 g, were randomly divided into three groups after successful preparation of a Langendorff isolated heart perfusion model with a stabilization period perfusion of 15 min with Krebs-Henseleit (K-H) fluid ( = 8): the control group (group A, continuously perfused with K-H fluid for 105 min), the ischemia-reperfusion group (group B, continuously perfused with K-H fluid for 15 min, and then exposed to 60 min of global ischemia induced by Thomas solution followed by 30 min of reperfusion), and the sevoflurane group (group C, K-H fluid contained 1.0 MAC sevoflurane, and other procedures were same as in group B). Heart rate (HR) and MAPs including time course (MAPD or MAPD) of the epicardium (Epi) and endocardium (Endo) were recorded at the time of balance perfusion for 15 min ( ), continuous perfusion for 15 min ( ), reperfusion for 15 min/continuous perfusion for 105 min ( ), and reperfusion for 30 min/continuous perfusion for 120 min ( ), and the transmural dispersion of repolarization (TDR) was calculated. The incidence of arrhythmia, time for restoration of spontaneous heart beat, and duration of arrhythmia were recorded during the period of reperfusion.
RESULTS
HR in group B and group C was lower at and than that in group A, while that in group B was significantly lower than that in group A at and , and HR in group C was higher than that in group B at and ( < 0.05). There was no difference of TDR in each group at and ( > 0.05), while TDR in group B was increased at and compared with that in group C and group A ( < 0.05). TDR in group C was decreased compared with that in group B at and ( < 0.05), while there was no such difference between group C and group A ( > 0.05). The time for restoration of spontaneous heart beat and duration of arrhythmia in group C were shorter than those in group B ( < 0.05), while cardiac arrhythmia scores in group B were higher than those in group C ( < 0.05). There was no difference of MAPD in each group ( > 0.05). The MAPD in group B was much longer than that in other groups at and ( < 0.05), while there was no such difference between group C and group A ( > 0.05). The prolonged MAPD at and in group B strikingly differed from that at and ( < 0.05). Nevertheless, there was no such difference in other groups at different time points ( > 0.05).
CONCLUSION
Sevoflurane alleviates reperfusion arrhythmia induced by myocardial ischemia-reperfusion through the shortening of MPAD in isolated rat hearts.
目的
探讨七氟醚对大鼠离体心脏缺血再灌注后单相动作电位（MAPs）的影响。
方法
24只健康SD雄性大鼠，体重280 - 320 g，成功制备Langendorff离体心脏灌注模型并经15分钟Krebs-Henseleit（K-H）液稳定灌注后（=8），随机分为三组：对照组（A组，持续用K-H液灌注105分钟）、缺血再灌注组（B组，持续用K-H液灌注15分钟，然后用Thomas液诱导全心缺血60分钟，再灌注30分钟）、七氟醚组（C组，K-H液含1.0 MAC七氟醚，其他步骤同B组）。在平衡灌注15分钟时（）、持续灌注15分钟时（）、再灌注15分钟/持续灌注105分钟时（）、再灌注30分钟/持续灌注120分钟时（）记录心率（HR）及包括心外膜（Epi）和心内膜（Endo）的单相动作电位时程（MAPD或MAPD），并计算复极跨壁离散度（TDR）。记录再灌注期间心律失常发生率、自主心跳恢复时间及心律失常持续时间。
结果
B组和C组在和时HR低于A组，而B组在和时HR显著低于A组，C组在和时HR高于B组（<0.05）。各组在和时TDR无差异（>0.05），而B组在和时TDR较C组和A组升高（<0.05）。C组在和时TDR较B组降低（<0.05），而C组和A组间无此差异（>0.05）。C组自主心跳恢复时间及心律失常持续时间短于B组（<0.05），而B组心律失常评分高于C组（<0.05）。各组MAPD无差异（>0.05）。B组在和时MAPD较其他组显著延长（<0.05），而C组和A组间无此差异（>0.05）。B组在和时延长的MAPD与在和时显著不同（<0.
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