College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, Jiangsu 215123, China.
Analyst. 2019 Dec 2;144(24):7351-7358. doi: 10.1039/c9an01608b.
An excellent spectral sensor for Hg2+ named 2-(2-((2-aminoethyl)thio)ethyl)-3',6'-bis(diethylamino)spiro[isoindoline-1,9'-xanthen]-3-one (RMTE) was achieved by a one-step reaction between rhodamine B and thiobisethylamine. RMTE could detect Hg2+ in nearly pure water reversibly and highly selectively, indicated by a new increasing absorption peak at 561 nm and 170-fold enhanced fluorescence at 578 nm coupled with remarkable visual and fluorescence color changes. When the Hg2+ concentration ([Hg2+]) varied from 0 to 120 μM, the absorbance and fluorescence intensity of the RMTE solution responded linearly to [Hg2+] with the detection limits of 2.08 and 0.14 μM, respectively. RMTE could work in ecologically and biologically favorable pH values of 6.41-8.33. The binding mode of RMTE toward Hg2+ was 1 : 1. RMTE could monitor Hg2+ in environmental water and living cells effectively with low cytotoxicity.
一种名为 2-(2-((2-氨乙基)硫代)乙基)-3',6'-双(二乙氨基)螺[异吲哚啉-1,9'-氧杂蒽]-3-酮 (RMTE) 的汞离子 (Hg2+) 光谱传感器，是通过罗丹明 B 和硫代双乙胺之间的一步反应合成的。RMTE 在近纯水相中能可逆且高选择性地检测 Hg2+，表现为在 561nm 处出现新的增强吸收峰，在 578nm 处荧光增强 170 倍，同时伴有明显的肉眼观察和荧光颜色变化。当 Hg2+浓度 ([Hg2+]) 从 0 到 120 μM 变化时，RMTE 溶液的吸光度和荧光强度与 [Hg2+]呈线性响应，检测限分别为 2.08 和 0.14 μM。RMTE 可以在生态和生物有利的 pH 值 6.41-8.33 范围内工作。RMTE 与 Hg2+的结合模式为 1:1。RMTE 具有低细胞毒性，能有效地在环境水样和活细胞中监测 Hg2+。
