Department of Orthopedics and Rehabilitation,Beijing Bo'ai Hospital,China Rehabilitation Research Center, School of Rehabilitation ,Capital Medical University, , 100068 beijing, China.
J Rehabil Med. 2020 Jan 2;52(1):jrm00001. doi: 10.2340/16501977-2622.
To identify differentially expressed serum proteins that could serve as sensitive biomarkers of heterotopic ossification in patients with traumatic brain injury.
From August 2014 to December 2015, 18 patients with traumatic brain injury were enrolled in the study, and blood samples were collected. Patients with traumatic brain injury were divided based on the presence (n=9 patients, heterotopic ossification group) or absence (n=9 patients, traumatic brain injury group or control group) of heterotopic ossification. Protein expression profiles were compared using 2-dimensional electrophoresis. Differentially expressed proteins were examined using matrix-assisted laser desorption/ionization and time-of-flight tandem mass spectrometry (MALDI-TOF/TOF). The differentially expressed proteins identified were further confirmed by Western blotting.
Seven protein spots were differentially expressed between heterotopic ossification and traumatic brain injury groups in 2-dimensional electrophoresis analysis. Vitamin D binding protein (Gc protein), retinol binding protein 4 (RBP4) and haptoglobin expression decreased significantly in the heterotopic ossification group compared with the control group (p < 0.05), and this was further confirmed by Western blotting.
Lower levels of expression of Gc protein, RBP4 and haptoglobin may be closely related to heterotopic ossification after traumatic brain injury. These proteins may be potential biomarkers of heterotopic ossification secondary to traumatic brain injury.
鉴定创伤性脑损伤患者异位骨化的敏感血清蛋白生物标志物。
2014 年 8 月至 2015 年 12 月，纳入 18 例创伤性脑损伤患者，采集血样。根据有无异位骨化（n=9 例，异位骨化组；n=9 例，创伤性脑损伤组或对照组）将创伤性脑损伤患者分为两组。采用二维电泳比较蛋白质表达谱。采用基质辅助激光解吸电离和飞行时间串联质谱（MALDI-TOF/TOF）检测差异表达蛋白。通过 Western 印迹进一步确认差异表达蛋白。
二维电泳分析显示，异位骨化组与创伤性脑损伤组之间有 7 个蛋白斑点差异表达。与对照组相比，异位骨化组维生素 D 结合蛋白（Gc 蛋白）、视黄醇结合蛋白 4（RBP4）和触珠蛋白的表达显著降低（p<0.05），Western 印迹进一步证实了这一点。
创伤性脑损伤后 Gc 蛋白、RBP4 和触珠蛋白表达水平降低可能与异位骨化密切相关。这些蛋白可能是创伤性脑损伤后异位骨化的潜在生物标志物。
