双波长激发单一荧光探针用于测定氨基酸浓度和对映体组成。
Excitation of One Fluorescent Probe at Two Different Wavelengths to Determine the Concentration and Enantiomeric Composition of Amino Acids.
机构信息
Department of Medicinal Chemistry, School of Pharmacy , Southwest Medical University , Luzhou 646000 , China.
Department of Chemistry , University of Virginia , Charlottesville , Virginia 22904 , United States.
出版信息
Org Lett. 2019 Nov 15;21(22):9036-9039. doi: 10.1021/acs.orglett.9b03437. Epub 2019 Oct 30.
When a novel fluorescent probe made of BINOL and coumarin is excited at λ = 365 nm in a neutral buffer solution, it shows fluorescence enhancement at 465 nm in the presence of an amino acid with a very small difference between the two enantiomers. When excited at λ = 467 nm, it shows highly enantioselective fluorescence enhancement at 534 nm. This allows the determination of both concentration and enantiomeric composition of amino acids.
当一种由 BINOL 和香豆素制成的新型荧光探针在中性缓冲溶液中于 λ = 365nm 激发时，在两种对映体之间差异非常小的氨基酸存在下，在 465nm 处显示出荧光增强。当在 λ = 467nm 激发时，在 534nm 处显示出高度对映选择性的荧光增强。这使得可以同时测定氨基酸的浓度和对映体组成。
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