Analytical & Environmental Science Division and Centralized Instrument Facility, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar, Gujarat, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.
Arch Microbiol. 2020 Mar;202(2):351-359. doi: 10.1007/s00203-019-01742-9. Epub 2019 Oct 30.
Coral-associated microbes from Marine National Park (MNP), Gulf of Kutch (GoK), Gujarat, India, were screened for siderophore production. Maximum siderophore-producing isolate NP-C49 and its compound were identified and characterized. The isolate was identified as Klebsiella sp. through 16S rRNA genes sequencing (GenBank accession nos. KY412519 and MTCC 25160). Antibiotic susceptibility profile against 20 commercial antibiotics showed its more sensitivity compared to human pathogenic strain, i.e., Klebsiella pneumonia. The compound was identified as phenazine-1-carboxylic acid (PCA) using the multinuclear ID (H and C) and 2D (H-H COSY and H-C HETCOR) NMR along with high-resolution mass spectrometry. No significant difference in the bacterial growth in the presence of PCA, FeCl and Fe(OH) indicated involvement of factors other than PCA in bacterial growth. The study first reports the identification and characterization of PCA from Klebsiella sp. both from terrestrial and marine sources.
从印度古吉拉特邦库奇湾海洋国家公园（MNP）的珊瑚相关微生物中筛选出产生铁载体的微生物。对产铁载体能力最强的分离株 NP-C49 及其化合物进行了鉴定和特性分析。通过 16S rRNA 基因测序（GenBank 登录号：KY412519 和 MTCC 25160），将该分离株鉴定为肺炎克雷伯氏菌。对 20 种商业抗生素的药敏谱分析显示，其对人类致病菌，即肺炎克雷伯氏菌的敏感性明显高于后者。该化合物通过多核 ID（H 和 C）和 2D（H-H COSY 和 H-C HETCOR）NMR 以及高分辨率质谱联用技术鉴定为吩嗪-1-羧酸（PCA）。PCA、FeCl 和 Fe(OH) 的存在对细菌生长没有显著影响，表明 PCA 以外的因素也参与了细菌的生长。本研究首次从陆地和海洋来源的肺炎克雷伯氏菌中鉴定和表征了 PCA。
