Zhou Enbo, Gong Shengyi, Hong Jiaxin, Feng Guoqiang
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, Chemical Biology Center, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan, 430079, PR China.
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, Chemical Biology Center, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan, 430079, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Feb 15;227:117657. doi: 10.1016/j.saa.2019.117657. Epub 2019 Oct 20.
A near-infrared (NIR) ratiometric fluorescent probe, NIR-Ratio-CO, was developed for rapid detection of carbon monoxide (CO) in both solution and living cells through the strategy of Pd-mediated Tsuji-Trost reaction. This probe shows a rapid, highly specific and sensitive detection process for CO, accompanied by colorimetric and distinct ratiometric fluorescence changes at 655 and 592 nm with a large Stokes shift up to 195 nm. The detection limit for CO was measured to be about 61 nM by the fluorescence method. In addition, this probe was successfully applied for ratiometric imaging of both exogenous and endogenous CO in living cells, indicating that it can be used as a novel tool for ratiometric fluorescent detection of CO in living systems.
通过钯介导的Tsuji-Trost反应策略，开发了一种近红外（NIR）比率荧光探针NIR-Ratio-CO，用于在溶液和活细胞中快速检测一氧化碳（CO）。该探针对CO表现出快速、高度特异性和灵敏的检测过程，伴随着比色变化以及在655和592 nm处明显的比率荧光变化，具有高达195 nm的大斯托克斯位移。通过荧光法测得CO的检测限约为61 nM。此外，该探针已成功应用于活细胞中外源性和内源性CO的比率成像，表明它可作为活细胞系统中CO比率荧光检测的新型工具。
