Rothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, UK.
Rothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, UK.
Food Chem. 2020 Mar 5;308:125652. doi: 10.1016/j.foodchem.2019.125652. Epub 2019 Oct 15.
Vegetables contain iron, zinc and phosphorus as complexes with phytates limiting their availability from a vegetarian diet, meaning non-haem iron deficiency anaemia and zinc deficiency immune malfunction are a risk. Although these elements have been analysed previously in biological fluids and cereal using LC-ICP-MS, there is no method suitable for analysing iron, zinc and phosphorus simultaneously in vegetables because of their complex matrix. In this study, we analysed iron, zinc and phosphorus in cabbage, broccoli, pepper, spinach, kale and rocket after a simulated gastrointestinal digestion using a newly optimised SEC-ICP-MS method. Ammonium nitrate, as the mobile phase, and a suitable rinsing regime, allowed good reproducibility and maintenance of the equipment. The method showed good reproducibility and can be easily adapted to other vegetables, as required.
蔬菜中含有铁、锌和磷，它们与植酸形成复合物，限制了素食中这些元素的生物利用率，这意味着非血红素铁缺乏性贫血和锌缺乏免疫功能障碍的风险。虽然这些元素之前已经在生物体液和谷物中使用 LC-ICP-MS 进行了分析，但由于其复杂的基质，没有适合同时分析蔬菜中铁、锌和磷的方法。在这项研究中，我们使用新优化的 SEC-ICP-MS 方法在模拟胃肠道消化后分析了卷心菜、西兰花、辣椒、菠菜、羽衣甘蓝和芝麻菜中的铁、锌和磷。作为流动相的硝酸铵和合适的冲洗方案，保证了良好的重现性和仪器的维护。该方法具有良好的重现性，并且可以根据需要轻松地应用于其他蔬菜。
