Taiwan Food and Drug Administration, 161-2 Kunyang St., Nangang Dist., Taipei City 11561, Taiwan.
Taiwan Food and Drug Administration, 161-2 Kunyang St., Nangang Dist., Taipei City 11561, Taiwan.
J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Nov 1;1130-1131:121788. doi: 10.1016/j.jchromb.2019.121788. Epub 2019 Sep 12.
Multilayer print designs are commonly used in commercial food packaging to attract consumers. UV-curable ink is generally used in this type of printing due to its ease of application, space saving, and rapid drying; however, there have been a number of health alerts related to the contamination of food by photoinitiators in UV-curable ink. In this study, we established a multi-analyte method by which to detect 30 photoinitiators simultaneously. We then applied this method to the analysis of five breakfast cereals and ten types of packaged juice to detect the presence of photoinitiator contamination. Sample treatment was performed using the QuEChERS (quick, easy, cheap, effective, rugged, and safe) method for the extraction of photoinitiators. Chromatographic separation of two isomers, methylbenzophenone (MBP) and isopropylthioxanthone (ITX), was achieved using a pentafluorophenyl propyl (PFP) column (1.7 µm, 100 × 2.1 mm i.d.) and MeOH: 5 mM formic acid-ammonium formate (pH 4.0) in gradient elution. The average recovery of photoinitiators from cereal was between 62.0 and 120.3%, with a coefficient of variation between 0.4 and 14.4%. The average recovery of photoinitiators from packaged juices was between 84.4 and 122.9% with a coefficient of variation between 0.5 and 9.5%. The contamination results were as follows: 13.1 ng/g triphenyl phosphate (TPP) was detected in one breakfast cereal, and 2-hydroxy-4-methoxy benzophenone (BP-3), 1-hydroxycyclohexyl phenyl-ketone (Irgacure 184), methyl-2-benzoylbenzoate (MOBB), and 2,4-diethyl-9H-thioxanthen-9-one (DETX) were detected in one of the packaged juices at levels ranging from 2.2 to 152.9 ng/g.
多层印刷设计常用于商业食品包装以吸引消费者。由于其易于应用、节省空间和快速干燥，因此通常在这种类型的印刷中使用紫外线固化油墨；然而，已经有一些与紫外线固化油墨中光引发剂污染食品有关的健康警报。在这项研究中，我们建立了一种同时检测 30 种光引发剂的多分析物方法。然后，我们将该方法应用于五种早餐麦片和十种包装果汁的分析中，以检测光引发剂污染的存在。使用 QuEChERS（快速、简单、廉价、有效、坚固和安全）方法进行样品处理，用于光引发剂的提取。使用五氟苯基丙基（PFP）柱（1.7 µm，100×2.1 mm i.d.）和 MeOH:5 mM 甲酸-甲酸铵（pH 4.0）在梯度洗脱下实现了两种异构体甲基苯甲酮（MBP）和异丙基噻吨酮（ITX）的色谱分离。从麦片获得的光引发剂的平均回收率在 62.0%至 120.3%之间，变异系数在 0.4%至 14.4%之间。从包装果汁中获得的光引发剂的平均回收率在 84.4%至 122.9%之间，变异系数在 0.5%至 9.5%之间。污染结果如下：在一种早餐麦片检测到 13.1ng/g 三苯基膦（TPP），在一种包装果汁中检测到 2-羟基-4-甲氧基二苯甲酮（BP-3）、1-羟基环己基苯基酮（Irgacure 184）、甲基-2-苯甲酰基苯甲酸酯（MOBB）和 2,4-二乙基-9H-噻吨-9-酮（DETX），浓度范围为 2.2 至 152.9ng/g。
