接触混合电位法用于现场和原位评估水果和蔬菜的抗氧化活性。

Contact hybrid potentiometric method for on-site and in situ estimation of the antioxidant activity of fruits and vegetables.

机构信息

Ural State University of Economics, 62/45, 8 Marta/Narodnoi Voli St., Yekaterinburg 620144, Russia; Ural Federal University named after the first President of Russia B. N. Yeltsin, 28, Mira St, Yekaterinburg 620078, Russia.

Ural State University of Economics, 62/45, 8 Marta/Narodnoi Voli St., Yekaterinburg 620144, Russia.

出版信息

Food Chem. 2020 Mar 30;309:125703. doi: 10.1016/j.foodchem.2019.125703. Epub 2019 Oct 21.

DOI:10.1016/j.foodchem.2019.125703

PMID:31670132

Abstract

A new version of the hybrid potentiometric method (HPM) for evaluating the antioxidant activity (AOA) of fruits and vegetables, which we called contact (CHPM), is proposed. A screen-printed electrode pair coated by a membrane impregnated with a mediator serves as a sensor system. The potential shift, which is observed when the sensor system is exposed to the sample slice is used as a source of information. CHPM provides reproducible and correct results: relative standard deviation does not exceed 8.4% and recovery of model antioxidant at close to 100%. The AOA values of fruits and vegetables found by CHPM are in the range of 1-21 μmol-eq/g. Correlation between the AOA of slices and suspensions is found. The proposed CHPM can be used in on-site and in situ formats.

摘要

我们提出了一种用于评估水果和蔬菜抗氧化活性（AOA）的混合电位法（HPM）的新版本，称为接触式（CHPM）。一个由涂有介质的膜包裹的印刷电极对用作传感器系统。当传感器系统暴露于样品切片时观察到的电位变化可作为信息源。CHPM 提供可重复且正确的结果：相对标准偏差不超过 8.4%，并且模型抗氧化剂的回收率接近 100%。通过 CHPM 发现的水果和蔬菜的 AOA 值在 1-21 μmol-eq/g 范围内。发现切片和悬浮液的 AOA 之间存在相关性。所提出的 CHPM 可用于现场和原位格式。