sp. nov., a novel endophytic actinobacterium isolated from bark of L.

A Gram-staining-positive, aerobic, rod-shaped, non-spore-forming actinobacterium, designated strain M2KJ-4, was isolated from a surface-sterilized bark of L. collected from Xinpu in Guizhou, PR China and characterized using a polyphasic approach to determine its taxonomic position. M2KJ-4 grew optimally with 1 % (w/v) NaCl at 25 °C and pH 8.0. Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested. Phylogenetic analysis based on 16S rRNA gene sequence indicated that M2KJ-4represented a member of the genus and shared the highest 16S rRNA gene sequence similarity with DSM 10552 (Z78209) (98.95 %). The DNA GC content of M2KJ-4 was 70.6 mol%. The average nucleotide identity value and estimated DDH value between M2KJ-4 and the type strain of were 86.1 % and 30.2 %, respectively. The cell-wall peptidoglycan contained ll-diaminopimelic acid and MK-9(H) was the predominant menaquinone. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids and unidentified lipids. The major fatty acids were C, 10-methyl C, C 2-OH and Cω9. On the basis of the results from phylogenetic, phenotypic and chemotaxonomic analysis, strain M2KJ-4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is M2KJ-4 (=KCTC 49174=CGMCC 1.13666).