Klebanov G I, Teselkin Iu O, Vladimirov Iu A
Biofizika. 1988 May-Jun;33(3):512-6.
Powerful antioxidant activity of human plasma was demonstrated by measuring the thiobarbituric acid reaction and Fe+2-induced chemiluminescence. Inhibition of lipid peroxidation was shown both for plasma lipids and for the suspension of egg lipoproteins, which was taken as a model system. The inhibitory effect of plasma peroxidation was removed by azide Na taken in the concentration of 0.5 mg/ml, but caeroplasmin activity in the plasma was completely suppressed at NaN3 concentration equal to 0.1 mg/ml. A low correlation (r = 0.75) between caeruloplasmin activity in the blood plasma and extent of chemiluminescence activation obtained in the presence of NaN3 was found. The presented data led to an assumption that only a part of lipid peroxidation inhibitors in the plasma can be attributed with caeruloplasmin.
通过测量硫代巴比妥酸反应和Fe+2诱导的化学发光，证明了人血浆具有强大的抗氧化活性。血浆脂质和作为模型系统的卵脂蛋白悬浮液均表现出脂质过氧化受到抑制。浓度为0.5mg/ml的叠氮化钠可消除血浆过氧化的抑制作用，但在NaN3浓度等于0.1mg/ml时，血浆中的铜蓝蛋白活性被完全抑制。发现血浆中铜蓝蛋白活性与在NaN3存在下获得的化学发光激活程度之间存在低相关性（r = 0.75）。所呈现的数据导致一种假设，即血浆中脂质过氧化抑制剂只有一部分可归因于铜蓝蛋白。
