State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
Science. 2019 Nov 15;366(6467). doi: 10.1126/science.aax7890. Epub 2019 Oct 31.
Gastrulation is a key event in embryonic development when the germ layers are specified and the basic animal body plan is established. The complexities of primate gastrulation remain a mystery because of the difficulties in accessing primate embryos at this stage. Here, we report the establishment of an in vitro culture (IVC) system that supports the continuous development of cynomolgus monkey blastocysts beyond early gastrulation up to 20 days after fertilization. The IVC embryos highly recapitulated the key events of in vivo early postimplantation development, including segregation of the epiblast and hypoblast, formation of the amniotic and yolk sac cavities, appearance of the primordial germ cells, and establishment of the anterior-posterior axis. Single-cell RNA-sequencing analyses of the IVC embryos provide information about lineage specification during primate early postimplantation development. This system provides a platform with which to explore the characteristics and mechanisms of early postimplantation embryogenesis in primates with possible conservation of cell movements and lineages in human embryogenesis.
原肠作用是胚胎发育过程中的一个关键事件，在此过程中胚胎的胚层被特化，基本的动物体模式被建立。由于在这个阶段获取灵长类胚胎存在困难，灵长类原肠作用的复杂性仍然是一个谜。在这里，我们报告了一种体外培养（IVC）系统的建立，该系统支持食蟹猴囊胚在受精后超过早期原肠作用阶段持续发育至 20 天。IVC 胚胎高度重现了体内早期附植后发育的关键事件，包括上胚层和下胚层的分离、羊膜腔和卵黄囊腔的形成、原始生殖细胞的出现以及前-后轴的建立。对 IVC 胚胎的单细胞 RNA 测序分析提供了关于灵长类早期附植后发育过程中谱系特化的信息。该系统为探索灵长类早期附植后胚胎发生的特征和机制提供了一个平台，同时可能有助于在人类胚胎发生中保留细胞运动和谱系。
