Shanghai Key Laboratory of Molecular Imaging , Shanghai University of Medicine and Health Sciences , Shanghai , 201318 , P. R. China.
School of Pharmacy , Fudan University , 826 Zhangheng Road , Shanghai , 201203 , P. R. China.
ACS Sens. 2019 Nov 22;4(11):3084-3087. doi: 10.1021/acssensors.9b01507. Epub 2019 Nov 12.
Recently, we published a paper on the detection of superoxide (O) with a water-soluble fluorescent probe (. , , 59-64), and Francesco Tampieri et al. provided comments on our publication, mostly on the detection medium (deionized water) we used. Herein we present our responses to the addressed questions to explain that although KO decomposes in aqueous environment, the results we obtained did not affect the general trend, since evidence from the literature afforded the correlation between KO in aqueous media as a surrogate of superoxide and enzymatically produced O for the probes wherein the deprotection pathway operated. Moreover, fluorescence imaging on cells and zebrafish embryos under PMA stimulation confirmed the effectiveness of our probe to detect superoxide using KO as a convenient source. The detailed studies from Francesco Tampieri and coauthors are scientifically meaningful for the reliable evaluation of fluorescent probes using KO as a surrogate of superoxide.
最近，我们发表了一篇关于使用水溶性荧光探针 (.,, 59-64) 检测超氧化物 (O) 的论文， Francesco Tampieri 等人对我们的论文提出了评论，主要是针对我们使用的检测介质（去离子水）。在此，我们对提出的问题做出回应，以解释尽管 KO 在水相环境中会分解，但我们得到的结果并没有影响总体趋势，因为文献中的证据表明，在以 KO 作为超氧化物替代物的水相介质中，以及在保护基团被去除的反应途径起作用的探针中，KO 与酶促产生的 O 之间存在相关性。此外，在 PMA 刺激下对细胞和斑马鱼胚胎进行荧光成像，证实了我们的探针使用 KO 作为方便的来源来检测超氧化物的有效性。Francesco Tampieri 及其同事的详细研究对于使用 KO 作为超氧化物替代物来可靠评估荧光探针具有重要的科学意义。
