Albuquerque Raphael P, Duarte Glauce C A, Duarte Nelise, Ohana Débora T, Santos Wilson C, Machado Thelma B
Programa de Pós-Graduação em Engenharia de Biossistemas, Universidade Federal Fluminense, Niterói, RJ, Brazil.
Programa de Pós-Graduação em Ciências Aplicadas a Produtos de Saúde, Faculdade de Farmácia, Universidade Federal Fluminense, Niterói, RJ, Brazil.
Nat Prod Res. 2021 Aug;35(15):2574-2578. doi: 10.1080/14786419.2019.1680662. Epub 2019 Nov 1.
Leaf extracts from are rich in pentacyclic triterpenic acids (PTAs), especially barbinervic acid (BA), which is an important biomarker of the species. Dichloromethane extracts of leaf samples harvested in Amazonian summer and winter seasons were analysed by infrared spectroscopy using ATR-FTIR technique aiming to evaluate barbinervic acid (BA) and its PTAs equivalent contents. A validated HPLC-DAD quantification method was also performed to compare the relationship between BA and PTAs contents in extracts with ATR-FTIR technique. The use of ATR-FTIR allowed a rapid, efficient and environment-friendly quantification method for total PTAs equivalent content, showing a significant statistical difference (< 0.05) in the production of these metabolites (38.66 μg/mL, summer; 13.62 μg/mL, winter). A mathematical correction factor between the HPLC-DAD and ATR-FTIR quantification methods was established.
[植物名称]的叶提取物富含五环三萜酸（PTA），尤其是巴氏神经酸（BA），它是该物种的重要生物标志物。采用衰减全反射傅里叶变换红外光谱（ATR-FTIR）技术，对在亚马逊夏季和冬季采集的[植物名称]叶样本的二氯甲烷提取物进行红外光谱分析，旨在评估巴氏神经酸（BA）及其五环三萜酸等效含量。还采用了经过验证的高效液相色谱-二极管阵列检测（HPLC-DAD）定量方法，以比较[植物名称]提取物中巴氏神经酸（BA）和五环三萜酸含量与ATR-FTIR技术之间的关系。ATR-FTIR的使用为总五环三萜酸等效含量提供了一种快速、高效且环保的定量方法，这些代谢物的产量显示出显著的统计学差异（<0.05）（夏季为38.66μg/mL；冬季为13.62μg/mL）。建立了HPLC-DAD和ATR-FTIR定量方法之间的数学校正因子。
