Programa de Pós-Graduação em Ciências Aplicadas a Produtos Para Saúde, Faculdade de Farmácia, Universidade Federal Fluminense, Rio de Janeiro, Brasil.
Facultad de Medicina, Departamento de Farmacología, Instituto Teófilo Hernando, Universidad Autónoma de Madrid, Madrid, Spain.
Nat Prod Res. 2021 Nov;35(22):4870-4875. doi: 10.1080/14786419.2020.1739675. Epub 2020 Mar 16.
We investigated the role of triterpene barbinervic acid from dichloromethane extract in vasopressor responses. Renal arteries were cannulated and perfused with Krebs-Hepes solution. Changes in aorta isometric tension were recorded and transferred to a data acquisition system. Cumulative curves were constructed based on the maximum effect of agonists. Barbinervic acid reduced the renal tonus induced by NA in a NO-dependent manner (IC = 30 μM). Triterpene (70 μM) also induced rapid and transient relaxation in aorta that had been precontracted with K+ (53.2 ± 0.05%) or phenylephrine (36.7 ± 0.05%). data revealed two possible active sites for interactions between barbinervic acid and NO synthase. Barbinervic acid showed a vasodilator effect and could potentially be used as a template for developing new molecules for the treatment of cardiovascular disease.
我们研究了来自二氯甲烷提取物的三萜巴宾尼酸在升压反应中的作用。用 Krebs-Hepes 溶液对肾动脉进行插管和灌流。记录主动脉等长张力的变化，并将其传输到数据采集系统。根据激动剂的最大效应构建累积曲线。巴宾尼酸以 NO 依赖的方式降低由 NA 诱导的肾张力（IC = 30μM）。三萜（70μM）也可诱导预先用 K+（53.2±0.05%）或苯肾上腺素（36.7±0.05%）收缩的主动脉快速和短暂松弛。数据显示巴宾尼酸与一氧化氮合酶相互作用的两个可能的活性位点。巴宾尼酸显示出血管舒张作用，可能可用作开发治疗心血管疾病的新分子的模板。
