Porter C D, Porter A G, Archard L C
Department of Biochemistry, Charing Cross and Westminster Medical School, London, UK.
Comput Appl Biosci. 1988 Apr;4(2):271-3. doi: 10.1093/bioinformatics/4.2.271.
Agarose gel electrophoresis to separate DNA molecules is a widely used technique in molecular biology but there is an upper limit to the sizes that can be resolved. Pulsed field techniques have extended this limit but require expensive equipment. Here we describe a home-made control unit to interface conventional electrophoresis equipment to a BBC microcomputer for the purposes of field inversion gel electrophoresis.
琼脂糖凝胶电泳用于分离DNA分子,是分子生物学中一项广泛应用的技术,但可分辨的DNA分子大小存在上限。脉冲场技术扩展了这一上限,但需要昂贵的设备。在此,我们描述了一种自制的控制单元,用于将传统电泳设备与BBC微型计算机连接,以进行场反转凝胶电泳。