Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon 97331
J Biol Chem. 2019 Nov 1;294(44):15898-15899. doi: 10.1074/jbc.H119.011335.
Ribonucleotide reductase (RNR) catalyzes the first committed reaction in DNA synthesis. Most of what we know about RNR regulation comes from studies with cultured cells and with purified proteins. In this study, Tran use technology to inactivate RNR large subunit expression in heart and skeletal muscle of mouse embryos. Analysis of these mutants paints a picture of dNTP regulation in whole animals quite different from that seen in studies of purified proteins and cultured cells.
核糖核苷酸还原酶（RNR）催化 DNA 合成的第一个关键反应。我们对 RNR 调控的了解大多来自于对培养细胞和纯化蛋白的研究。在这项研究中，Tran 使用基因敲除技术使小鼠胚胎心脏和骨骼肌中的 RNR 大亚基表达失活。对这些突变体的分析描绘了一幅与在纯化蛋白和培养细胞研究中观察到的完全不同的整体动物 dNTP 调控图景。
