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传统发酵牦牛乳的微生物多样性和挥发性成分分析。

Microbial diversity and volatile profile of traditional fermented yak milk.

机构信息

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy and Food Co. Ltd., Shanghai 200436, China.

出版信息

J Dairy Sci. 2020 Jan;103(1):87-97. doi: 10.3168/jds.2019-16753. Epub 2019 Oct 31.

Abstract

Previous research reported that fermented yak milk had a diverse microbial composition. For this study, raw yak milk, qula, and fermented yak milk samples were collected from the Aba Tibetan autonomous region of China. The genus and species microbial composition of these samples were analyzed by high-throughput sequencing of 16S rRNA and groEL gene amplicons, and the volatile profile of the samples was quantified by gas chromatography-mass spectrometry. The results indicated variation in abundance of microbiota at the genus level among the fermented yak milk samples, with Lactobacillus as the most abundant genus in the majority of samples, ranging from 41.6 to 98.3%. The volatile profile of the samples varied among those collected from different villages. Correlations between bacterial composition and volatile compounds of the samples were also observed. Lactobacillus displayed a significant correlation with volatile compounds such as benzaldehyde, 2,3-pentanedione, ethanol, and ethyl acetate, whereas the samples with relatively high abundance of Streptococcus and Lactococcus displayed relatively low contents of volatile compounds.

摘要

先前的研究报告称,发酵牦牛乳具有多样的微生物组成。在这项研究中,从中国阿坝藏族羌族自治州采集了生牦牛乳、曲拉和发酵牦牛乳样本。通过高通量测序 16S rRNA 和 groEL 基因扩增子分析了这些样本的属和种微生物组成,并通过气相色谱-质谱法对样本的挥发性成分进行了定量分析。结果表明,发酵牦牛乳样本在属水平上的微生物丰度存在差异,其中乳杆菌是大多数样本中最丰富的属,丰度范围为 41.6%至 98.3%。不同村庄采集的样本挥发性成分也存在差异。还观察到了细菌组成和样本挥发性化合物之间的相关性。乳杆菌与苯甲醛、2,3-戊二酮、乙醇和乙酸乙酯等挥发性化合物呈显著相关,而链球菌和乳球菌含量较高的样本挥发性化合物含量较低。

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