Mohamed Magdy M, Eissa Sanaa, Mostafa Mona, Hegazy Marwa G A
1Biochemistry Department, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt.
2Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Indian J Clin Biochem. 2019 Oct;34(4):472-478. doi: 10.1007/s12291-018-0773-7. Epub 2018 Jun 22.
Autophagy is a highly conserved pathway. Impairment of autophagy is implicated in the pathogenesis of diabetic nephropathy. The current study applied a bioinformatics analysis to retrieve promising autophagy biomarker relevant diabetic nephropathy. Urinary expression of Microtubule-associated protein 1 light-chain 3B (LC3B) RNA was assessed. Urine samples of 86 type II diabetic kidney disease Egyptian patients (albuminuria group) were provided to quantify urinary expression of LC3B. A group of 30 healthy volunteers were also enrolled in addition to non-albuminuria group including 44 patients. Our study revealed a cut-off value for urinary LC3B expression level that was calculated by receiver-operating characteristic curve as 0.866. Sensitivity and specificity of LC3B were 83.7 and 78.4% respectively. The positivity rate of urinary LC3B expression level was significantly lower in diabetic nephropathy patients than control group. LC3B has great clinical value as promising biomarker in diabetic nephropathy assessment.
自噬是一条高度保守的途径。自噬功能受损与糖尿病肾病的发病机制有关。当前研究应用生物信息学分析来检索与糖尿病肾病相关的有前景的自噬生物标志物。评估了微管相关蛋白1轻链3B（LC3B）RNA的尿液表达。提供了86例埃及II型糖尿病肾病患者（蛋白尿组）的尿液样本以定量LC3B的尿液表达。除了包括44例患者的非蛋白尿组外，还招募了30名健康志愿者。我们的研究通过受试者工作特征曲线计算出尿液LC3B表达水平的临界值为0.866。LC3B的敏感性和特异性分别为83.7%和78.4%。糖尿病肾病患者尿液LC3B表达水平的阳性率显著低于对照组。LC3B作为有前景的生物标志物在糖尿病肾病评估中具有重要的临床价值。
