Garland Jack, Philcox Winston, McCarthy Sinead, Mathew Suneeth, Hensby-Bennett Sarah, Ondrushka Benjamin, Woydt Lina, Da Broi Ugo, Palmiere Cristian, Lam Leo, Ahn Yeri, Olds Kelly, Glenn Charley, Morrow Paul, Kesha Kilak, Stables Simon, Tse Rexson
From the Forensic and Analytical Science Service, NSW Health Pathology, New South Wales, Australia.
School of Medicine, Faculty of Medical and Health Sciences, University of Auckland.
Am J Forensic Med Pathol. 2019 Dec;40(4):351-355. doi: 10.1097/PAF.0000000000000515.
Postmortem tryptase is a useful biochemical test to aid the diagnosis of anaphylaxis. Multiple perimortem and postmortem factors have been documented to cause an elevation in postmortem tryptase level. One factor that was recently recognized to have an impact on postmortem tryptase level is correct sampling technique. A recent study recommended aspirating blood samples from a clamped femoral/external iliac vein to be used for reliable postmortem tryptase analysis. This study sampled 120 consecutive nonanaphylactic deaths in which all the peripheral bloods were sampled as recommended. Postmortem interval, resuscitation, different nonanaphylactic causes of death, sex, and age did not show any statistical significant relation to postmortem tryptase level in Student t test, Pearson correlation, and univariate and multivariate analyses. The mean (SD) postmortem tryptase level was 8.4 (5.2) μg/L (minimum, 1.0 μg/L; maximum, 36.1 μg/L; median, 7.3 μg/L). Using nonparametric methods, the postmortem tryptase reference range in nonanaphylactic death was established as <23 μg/L (97.5th percentile).
尸检组织蛋白酶是有助于诊断过敏反应的一项有用的生化检测。多项濒死期和死后因素已被证明会导致尸检组织蛋白酶水平升高。最近认识到对尸检组织蛋白酶水平有影响的一个因素是正确的采样技术。最近一项研究建议从钳夹的股静脉/髂外静脉采集血样用于可靠的尸检组织蛋白酶分析。本研究对120例连续的非过敏性死亡病例进行采样，所有外周血均按建议采集。在学生t检验、Pearson相关性分析以及单变量和多变量分析中，死后间隔、复苏情况、不同的非过敏性死亡原因、性别和年龄与尸检组织蛋白酶水平均未显示出任何统计学上的显著相关性。尸检组织蛋白酶水平的均值（标准差）为8.4（5.2）μg/L（最小值，1.0μg/L；最大值，36.1μg/L；中位数，7.3μg/L）。使用非参数方法，非过敏性死亡中尸检组织蛋白酶的参考范围确定为<23μg/L（第97.5百分位数）。
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