Forensic Medicine and Coroners Court Complex, NSW Health Pathology, Australia.
Institute of Legal Medicine, University Medical Centre Hamburg-Eppendorf, Germany.
Med Sci Law. 2021 Oct;61(4):250-255. doi: 10.1177/0025802421998851. Epub 2021 Mar 2.
Haemolysis is reported to be an artefact that may alter post-mortem tryptase levels. However, previous studies did not sample peripheral blood using newly standardised methods. Recent studies have shown that some previously recognised peri- and post-mortem confounders can be muted by careful sample collection with first clamping and then sampling the femoral vein. This prospective study investigated the relationship between the degree of haemolysis of the blood samples and femoral vein post-mortem tryptase levels when sampled using this recommended method. Seventy consecutive post-mortem tryptase levels in non-anaphylactic deaths were compared to the degree of haemolysis of these samples, and results showed no significant correlation between them. The mean post-mortem tryptase level was 9.5 μg/L. This study demonstrated that the effects of haemolysis on femoral vein post-mortem tryptase was negligible when the blood was sampled using the recommended sampling method. Future studies on post-mortem tryptase as well as other typically used blood markers in forensics are recommended to adopt this method of blood sampling in routine practice.
溶血被报道为一种可能改变死后胰蛋白酶水平的人为假象。然而，以前的研究并没有使用新的标准化方法对外周血进行采样。最近的研究表明，一些先前被认为是围死亡期的混杂因素，可以通过小心地采集样本来消除，首先夹住股静脉，然后再从股静脉中抽取样本。本前瞻性研究调查了使用这种推荐方法采样时，血液样本溶血程度与股静脉死后胰蛋白酶水平之间的关系。将 70 例非过敏性死亡的连续死后胰蛋白酶水平与这些样本的溶血程度进行比较，结果显示两者之间无显著相关性。死后胰蛋白酶的平均水平为 9.5μg/L。本研究表明，当使用推荐的采样方法采集血液时，溶血对股静脉死后胰蛋白酶的影响可以忽略不计。建议未来关于死后胰蛋白酶以及法医学中其他常用血液标志物的研究在常规实践中采用这种血液采样方法。
