State Key Laboratory for Quality and Safety of Agro-Products, Ningbo University, Ningbo, 315211, China.
School of Marine Science, Ningbo University, Ningbo, China.
Arch Microbiol. 2020 Apr;202(3):437-445. doi: 10.1007/s00203-019-01759-0. Epub 2019 Nov 5.
Vibrio parahemolyticus is a halophilic bacterium which causes widespread seafood poisoning pathogenicity. Although the incidence of disease caused by V. parahemolyticus was stepwise increased, the pathogenic mechanism remained unclear. Herein, the difference of V. parahemolyticus's metabonomic which on blood agar and seawater beef extract peptone medium was detected via nuclear magnetic resonance and 55 metabolites were identified. Among them, 40 kinds of metabolites were upregulated in blood agar group, and 12 kinds were downregulated. Nine pathways were verified by enrichment analysis which were predicted involved in amino acids and protein synthesis, energy metabolism, DNA and RNA synthesis and DNA damage repair. We supposed that the metabolic pathway obtained from this study is related to V. parahemolyticus pathogenicity and our findings will aid in the identification of alternative targets or strategies to treat V. parahemolyticus-caused disease.
副溶血性弧菌是一种嗜盐菌，可引起广泛的食源性海产品中毒致病性。尽管由副溶血性弧菌引起的疾病发病率逐渐增加，但发病机制尚不清楚。在此，通过核磁共振检测了在血琼脂和海水牛肉浸粉培养基上副溶血性弧菌的代谢组学差异，共鉴定出 55 种代谢物。其中，血琼脂组上调 40 种代谢物，下调 12 种代谢物。通过富集分析验证了 9 条途径，预测这些途径涉及氨基酸和蛋白质合成、能量代谢、DNA 和 RNA 合成以及 DNA 损伤修复。我们推测，从本研究中获得的代谢途径与副溶血性弧菌的致病性有关，我们的研究结果将有助于鉴定治疗副溶血性弧菌引起的疾病的替代靶标或策略。
