Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland.
Microb Cell Fact. 2023 Sep 28;22(1):197. doi: 10.1186/s12934-023-02206-y.
Ovarian cancer is one of the most frequent and deadly gynaecological cancers, often resistant to platinum-based chemotherapy, the current standard of care. Halophilic microorganisms have been shown to produce a large variety of metabolites, some of which show toxicity to various cancer cell lines. However, none have yet been shown to be active against ovarian cancer cells. Here, we examined the effects of metabolites secreted by the halophilic archaea Halorhabdus rudnickae and Natrinema salaciae on various cancer cell lines, including ovarian cancer cell lines.
H NMR analyses of Hrd. rudnickae and Nnm. salaciae culture supernatants contain a complex mixture of metabolites that differ between species, and even between two different strains of the same species, such as Hrd. rudnickae strains 64 and 66. By using the MTT and the xCELLigence RTCA assays, we found that the secreted metabolites of all three halophilic strains expressed cytotoxicity to the ovarian cancer cell lines, especially A2780, as well as its cisplatin-resistant derivative A2780cis, in a dose-dependent manner. The other tested cell lines A549, HepG2, SK-OV-3 and HeLa were only minimally, or not at all affected by the archaeal metabolites, and this was only seen with the MTT assay.
The halophilic archaea Hrd. rudnickae and Nnm. salaciae, isolated from a Polish salt mine and Lake Medee in the Mediterranean Sea, respectively, secrete metabolites that are active against ovarian cancer cells, including those that are resistant to cisplatin. This opens potential new possibilities for the treatment of these frequent and deadly gynaecological cancers.
卵巢癌是最常见和最致命的妇科癌症之一，通常对基于铂的化疗（当前的标准治疗方法）具有耐药性。嗜盐微生物已被证明能产生大量代谢物，其中一些对各种癌细胞系具有毒性。然而，目前还没有证据表明它们对卵巢癌细胞具有活性。在这里，我们研究了嗜盐古菌 Halorhabdus rudnickae 和 Natrinema salaciae 分泌的代谢物对各种癌细胞系（包括卵巢癌细胞系）的影响。
Hrd. rudnickae 和 Nnm. salaciae 培养上清液的 1 H NMR 分析包含代谢物的复杂混合物，这些代谢物在物种之间甚至在同一物种的两个不同菌株之间都有所不同，例如 Hrd. rudnickae 菌株 64 和 66。通过使用 MTT 和 xCELLigence RTCA 测定法，我们发现三种嗜盐菌株分泌的代谢物均对卵巢癌细胞系表现出细胞毒性，尤其是 A2780 及其顺铂耐药衍生物 A2780cis，且具有剂量依赖性。其他测试的细胞系 A549、HepG2、SK-OV-3 和 HeLa 仅受到轻微影响，或者根本不受古菌代谢物的影响，而这仅在 MTT 测定法中可见。
分别从波兰盐矿和地中海 Medee 湖中分离出来的嗜盐古菌 Hrd. rudnickae 和 Nnm. salaciae 分泌的代谢物对卵巢癌细胞具有活性，包括对顺铂耐药的细胞。这为治疗这些常见且致命的妇科癌症开辟了新的可能性。
