Jalava A M, Heikkilä J E, Akerman K E
Department of Biochemistry and Pharmacy, Abo Academy, Finland.
Exp Cell Res. 1988 Nov;179(1):10-7. doi: 10.1016/0014-4827(88)90343-6.
The induction of differentiation in SH-SY5Y human neuroblastoma cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) is accompanied by a rapid and a transient expression of c-fos mRNA and a down-regulation of c-myc mRNA. The TPA-induced expression of c-fos mRNA was inhibited by H-7, a specific inhibitor of protein kinase C (PK-C). Dioctanoylglycerol (DiC8) failed to induce differentiation of SH-SY5Y cells or to down-regulate c-myc mRNA but it did induce the expression of c-fos mRNA. Treatment of IMR-32 human neuroblastoma cells with TPA did not cause differentiation although c-fos mRNA was induced. Since PK-C in SH-SY5Y cells was activated by both TPA and DiC8 it is suggested that the activation of PK-C alone is not sufficient to induce differentiation in SH-SY5Y cells. The down-regulation of c-myc mRNA rather than the induction of c-fos mRNA seems to be associated with differentiation process in SH-SY5Y cells.
用12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）诱导SH - SY5Y人神经母细胞瘤细胞分化，伴随着c - fos mRNA的快速短暂表达以及c - myc mRNA的下调。蛋白激酶C（PK - C）的特异性抑制剂H - 7抑制了TPA诱导的c - fos mRNA表达。二辛酰甘油（DiC8）未能诱导SH - SY5Y细胞分化或下调c - myc mRNA，但它确实诱导了c - fos mRNA的表达。用TPA处理IMR - 32人神经母细胞瘤细胞虽诱导了c - fos mRNA，但未引起分化。由于TPA和DiC8均可激活SH - SY5Y细胞中的PK - C，因此提示仅PK - C的激活不足以诱导SH - SY5Y细胞分化。c - myc mRNA的下调而非c - fos mRNA的诱导似乎与SH - SY5Y细胞的分化过程相关。
