Kolodrubetz D, Haggren W, Burgum A
Department of Microbiology, University of Texas Health Science Center, San Antonio 78284.
FEBS Lett. 1988 Sep 26;238(1):175-9. doi: 10.1016/0014-5793(88)80251-5.
Several nonhistone chromatin proteins (NHPs) have been isolated from Saccharomyces cerevisiae nuclei. They have molecular masses and amino acid compositions typical of the high mobility group (HMG) proteins from higher eukaryotic cells. Polyclonal antisera raised against two of the NHPs have been used in immunoblots of proteins from subcellular fractions of yeast to show that the NHPs are indeed nuclear. In addition, the amino-terminal amino acid sequences of several of the NHPs were determined. Importantly, the amino-terminal sequence of one of the proteins, NHP6, has significant (60%) identity with a stretch of amino acids in calf thymus HMG1.
已从酿酒酵母细胞核中分离出几种非组蛋白染色质蛋白（NHP）。它们具有来自高等真核细胞的高迁移率族（HMG）蛋白典型的分子量和氨基酸组成。针对其中两种NHP产生的多克隆抗血清已用于酵母亚细胞组分蛋白质的免疫印迹分析，以表明这些NHP确实存在于细胞核中。此外，还测定了几种NHP的氨基末端氨基酸序列。重要的是，其中一种蛋白质NHP6的氨基末端序列与小牛胸腺HMG1中的一段氨基酸具有显著的（60%）同源性。
