Miyamoto S, Fujime S
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
FEBS Lett. 1988 Sep 26;238(1):67-70. doi: 10.1016/0014-5793(88)80226-6.
In a range of [Ca2+] similar to cytosolic transient, a drastic reduction from about 20 dyn/cm to almost zero was observed in the membrane elastic modulus of bovine chromaffin granules, isolated in a solution containing 0.3 M sucrose and 5 mM Hepes at pH 7.0, and measured by combination of osmotic swelling and dynamic light-scattering (DLS) methods. This result suggests that the granule membrane becomes extremely flexible as a prelude to exocytosis.
在与胞质瞬变相似的[Ca2+]范围内，在含有0.3 M蔗糖和5 mM Hepes（pH 7.0）的溶液中分离并通过渗透压肿胀和动态光散射（DLS）方法组合测量的牛嗜铬粒蛋白颗粒的膜弹性模量，从约20达因/厘米急剧降低至几乎为零。该结果表明，颗粒膜在胞吐作用之前变得极其柔韧。
