从仙人掌Machaerocereus eruca中纯化两种凝集素并进行部分特性分析。

Purification and partial characterization of two lectins from the cactus Machaerocereus eruca.

作者信息

Zenteno E, Debray H, Montreuil J

机构信息

Laboratoire de Chimie Biologique de l'Université des Sciences et Techniques de Lille Flandres-Artois (Unité Associée au Centre National de La Recherche Scientifique no. 217, Villeneuve d'Ascq, France.

出版信息

FEBS Lett. 1988 Sep 26;238(1):95-100. doi: 10.1016/0014-5793(88)80233-3.

DOI:10.1016/0014-5793(88)80233-3

PMID:3169259

Abstract

Two lectins (MEAI and MEAII) were isolated from the cactus Machaerocereus eruca by affinity chromatography on mucin-Sepharose and partially characterized with respect to their biochemical and carbohydrate binding properties. Both are oligomeric glycoproteins consisting of 35 kDa monomers. Amino acid analysis indicates that both lectins have similar composition with high amounts of glycine, glutamic acid and serine. MEAI and MEAII contain approximately 36 and 24% (w/w) of carbohydrates, respectively. They agglutinate erythrocytes from several animal species. Binding specificity was directed to galactose-containing oligosaccharides and glycopeptides. The M. eruca lectins are the first lectins to be isolated from a species belonging to the plant family of Cactaceae.

摘要

通过在粘蛋白-琼脂糖上进行亲和层析，从仙人掌毛花柱中分离出两种凝集素（MEAI和MEAII），并对其生化和碳水化合物结合特性进行了部分表征。两者均为寡聚糖蛋白，由35 kDa单体组成。氨基酸分析表明，两种凝集素组成相似，含有大量的甘氨酸、谷氨酸和丝氨酸。MEAI和MEAII分别含有约36%和24%（w/w）的碳水化合物。它们能凝集多种动物物种的红细胞。结合特异性针对含半乳糖的寡糖和糖肽。毛花柱凝集素是从仙人掌科植物中分离出的首批凝集素。