通过密度梯度离心和逆流离心淘析法分离太平洋牡蛎血细胞。
Separation of Crassostrea gigas hemocytes by density gradient centrifugation and counterflow centrifugal elutriation.
作者信息
Bachère E, Chagot D, Grizel H
机构信息
IFREMER, Laboratoire de pathologie et de Génétique des Invertébrés Marins, La Tremblade, France.
出版信息
Dev Comp Immunol. 1988 Summer;12(3):549-59. doi: 10.1016/0145-305x(88)90071-7.
PMID:3169352
Abstract
A protocol is described to separate several subpopulations of hemocytes in a unique medium which avoids cell aggregation and retains cell-viability. Isopycnic centrifugation in Percoll followed by counterflow centrifugal elutriation provides large quantities of separated granulocyte and hyalinocyte subpopulations.
摘要
本文描述了一种在独特培养基中分离血细胞亚群的方案，该培养基可避免细胞聚集并保持细胞活力。采用Percoll等密度离心，随后进行逆流离心淘析，可得到大量分离的粒细胞和透明细胞亚群。
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