莫西沙星可抑制哮喘大鼠气道平滑肌细胞的气道炎症，并调节小窝蛋白-1和小窝蛋白-1的表达。

Moxifloxacin suppresses airway inflammation and modulates expression of caveolin-1 and flotillin-1 in airway smooth muscle cells of asthmatic rats.

作者信息

Li Hui-Ting, Ye Cong, Zhou Min, Yang Yan, Jin Quan, Pan Chun-Feng

机构信息

Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, Tongji University School, Shanghai 200433, China.

Department of Respiratory Medicine, Shanghai Jinshan Central Hospital, Shanghai 201500, China.

出版信息

Ann Transl Med. 2019 Sep;7(18):469. doi: 10.21037/atm.2019.08.43.

DOI:10.21037/atm.2019.08.43

PMID:31700905

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6803200/

Abstract

BACKGROUND

Moxifloxacin (MXF) possesses anti-inflammatory properties on asthmatic airway smooth muscle cells (ASMCs) beyond their antimicrobial effects, but the mechanisms are still unknown. This study was to investigate effects of MXF on expression of caveolin-1 (Cav-1) and flotillin-1 (FLOT1) in ASMCs in asthmatic rats.

METHODS

ASMCs were collected from the airway and cultured . Cells from normal rats were treated with normal saline (Group N); cells from asthmatic rats were incubated with normal saline (Group A) or MXF (20 mg/L) (Group M); Cav-1 expression was up-regulated by transferring Cav-1 expressing lentivirus (Group L) and FLOT1 expression down-regulated by using siRNA in cells from asthmatic rats (Group S). The expressions of Cav-1, FLOT1 and p65 NF-κB were measured by Western blotting and quantificational real-time polymerase chain reaction (qRT-PCR), and interleukin-8 (IL-8) and eotaxin contents were measured by enzyme-linked immunosorbent assay (ELISA).

RESULTS

Compared with normal control, Cav-1 expression significantly decreased in asthmatic groups (P<0.01); MXF up-regulated Cav-1 expression in asthmatic groups (P<0.01). However, compared with normal control, the expression of FLOT1 and p65 NF-κB dramatically increased in asthmatic groups (P<0.01); MXF down-regulated the expression of FLOT1 and p65 NF-κB in asthmatic groups (P<0.01); meanwhile, the expressions of FLOT1 and p65 NF-κB decreased after up-regulation of Cav-1 expression in asthmatic groups (P=0.01). Compared with asthmatic groups, the IL-8 and eotaxin contents significantly decreased in MXF Groups, Cav-1 up-regulation asthmatic groups and FLOT1 down-regulation asthmatic groups (P<0.01).

CONCLUSIONS

MXF can modulate the airway inflammation, upregulate Cav-1 expression, downregulate the expression of FLOT1 and p65 NF-κB in asthmatic rat ASMCs, which may be related to the anti-inflammatory effects of MXF in asthmatic ASMCs.

摘要

背景

莫西沙星（MXF）对哮喘气道平滑肌细胞（ASMCs）具有抗菌作用之外的抗炎特性，但其机制尚不清楚。本研究旨在探讨MXF对哮喘大鼠ASMCs中小窝蛋白-1（Cav-1）和浮舰蛋白-1（FLOT1）表达的影响。

方法

从气道收集ASMCs并进行培养。正常大鼠的细胞用生理盐水处理（N组）；哮喘大鼠的细胞用生理盐水孵育（A组）或用MXF（20mg/L）孵育（M组）；通过转染表达Cav-1的慢病毒上调Cav-1表达（L组），并在哮喘大鼠细胞中使用小干扰RNA下调FLOT1表达（S组）。通过蛋白质免疫印迹法和定量实时聚合酶链反应（qRT-PCR）检测Cav-1、FLOT1和p65核因子κB（NF-κB）的表达，通过酶联免疫吸附测定（ELISA）检测白细胞介素-8（IL-8）和嗜酸性粒细胞趋化因子含量。

结果

与正常对照组相比，哮喘组Cav-1表达显著降低（P<0.01）；MXF上调哮喘组Cav-1表达（P<0.01）。然而，与正常对照组相比，哮喘组FLOT1和p65 NF-κB表达显著增加（P<0.01）；MXF下调哮喘组FLOT1和p65 NF-κB表达（P<0.01）；同时，哮喘组上调Cav-1表达后FLOT1和p65 NF-κB表达降低（P=0.01）。与哮喘组相比，MXF组、Cav-1上调哮喘组和FLOT1下调哮喘组的IL-8和嗜酸性粒细胞趋化因子含量显著降低（P<0.01）。